创伤患者血浆内毒素与抵抗素的变化及意义

Changes and significance of lipopolysaccharide and resistin in serum of patients with trauma

目的探讨创伤患者血浆内毒素（LPS）和抵抗素的变化规律及其临床意义。方法采用基质显色法和酶联免疫吸附法（ELISA），动态检测70例创伤患者伤后1周内血浆LPS和抵抗素的水平，并分析抵抗素水平与患者LPS水平、伤情及严重并发症的关系。结果创伤患者血中LPS和抵抗素于第3天达到高峰，其中多器官功能障碍综合征（MODS）组LPS和抵抗素含量较非MODS组高（P〈0．05）；血浆抵抗素和LPS水平呈正相关（r=0．645，P〈0．05）。结论创伤患者伤后血浆LPS水平升高可能是抵抗素升高的重要因素，抵抗素的变化与患者的免疫机能及并发症密切相关。

Objective To detect the changes of lipopolysaccharide （LPS） and resistin in serum of patients with trauma. Methods The level of LPS and resistin were measured with chromogenic substrate method and enzyme linked immunosorbent assay （ELISA） in 70 patients with trauma during a week after trauma. The relationship was analyzed between the levels of LPS and resistin and complications. Results LPS and resistin reached the peak at posttranma day 3, which were more significant in patients with MODS as compared with the patients with out MODS（ P 〈 0.05 ）. In addition, resistin was correlated with LPS level （r = 0. 645, P 〈 0. 05）. Conclusions LPS might play an important role in the change of ratio of resistin that is closely correlated with the immunological function and complication after severe trauma.