脑膜炎奈瑟菌荧光定量PCR法检测

Application of real-time PCR for detection of Neisseria meningitides

目的评价新型水解探针(TaqMan-MGB探针)荧光定量PCR法在检测脑膜炎奈瑟菌中的应用价值。方法分别采用TaqMan-MGB探针荧光定量PCR法、常规PCR法和传统分离培养法检测230例无症状者咽拭子标本,比较3种方法的检出率。结果3种方法的阳性检出率分别为10.43%,6.52%和3.48%;TaqMan-MGB探针荧光定量PCR法的检出率高于常规PCR法和传统分离培养法(P<0.001)。结论TaqMan-MGB探针荧光定量PCR法检测脑膜炎奈瑟菌具有灵敏、特异、快速方便的特点,可为流脑流行病学调查提供一种快速、方便的病原学诊断手段,具有实用价值。

Objective To evaluate the application value of a new type probe（ TaqMan-MGB probe） in real-time PCR for detection of Neisseria meningitides. Methods Overall 230 samples of throat swabs from healthy people were detected with the established TaqMan-MGB real-time PCR, simple PCR and conventional methods. The detection rates of the three methods were compared to analyze their differences. Results The positive rate of Neisseria meningitides detected by Taq- Man-MGB probe real-time PCR （ 10. 47 % ） was significantly higher than conventional methods （3.48%） and simple PCR （6. 25%, P 〈 0. 001 ）. Condusion The TaqMan-MGB probe real-time PCR is a rapid, high sensitive and specific method in the detection of Neisseria meningitides, which could provide a rapid and convenient etiological diagnosis method for Neisseria meningitides in epidemiological investigation.