口腔鳞癌亲本细胞株与耐药细胞株蛋白谱差异

Differential protein expression between oral squanmous carcinoma parental cells and multidrug resistance cells in vitro

目的探讨人口腔鳞癌体外培养亲本细胞株KB与耐药细胞株KBV200的蛋白质表达差异。方法在体外培养KB及KBV200细胞株,采用表面增强激光解析电离飞行时间质谱技术(SELDI)及蛋白质芯片技术检测KB及KBV200蛋白质谱。结果体外培养的口腔鳞癌亲本细胞株与耐药细胞株的蛋白质存在差异表达,CM-10芯片共捕获102个蛋白,发现差异峰19个,其中15个差异蛋白在KBV200细胞中高表达,4个差异蛋白在KBV200细胞中低表达。结论SELDI蛋白芯片技术检测口腔鳞癌亲本细胞株与耐药细胞株蛋白质的差异表达方法简便、敏感、重复性好。

Objective To investigate differential protein expressions between oral squamous carcinoma cell line KB and multidrug resistance cell line KBV200 in vitro. Methods The surface enhanced laser desorption ionization（SELDI） was used. The KB and KBV200 cells were harvested and then split by ceil lysate solution. The protein mass spectra of the KB and KBV200 cells was detected by CM-10 chip based on SELDI protein chip technology. Results A total of 102 proteins were captured by CM-10 chip,and 19 differential peaks were found. Among those differentially expressed proteins, 15 were highly expressed, while 5 were lowly expressed in KBV200 cells. Conclusion SELDI technology is convenient, sensitive, and repeatable in the detection of the different proteins expressions between oral squamous carcinoma cell line KB and multi- drug resistance cell line KBV200.