不同生物实验室气载真菌检测分析

Potential harm of fungal aerosol in scientific research environment

目的分析实验室真菌气溶胶分布特征,为生物实验室工作人员提供预警资料。方法采用6级An-derson空气采样器,马铃薯葡萄糖琼脂(PDA)培养基分别在真菌实验室,普通实验室进行采样。结果真菌实验室的优势菌株为黑曲霉、岛青霉和黄曲霉,分别高于平均浓度195,60和20 CFU/m3;普通实验室的优势菌株为青霉,含量高达67.4%。真菌实验室、普通实验室气溶胶浓度分别为813和304 CFU/m3,计数中值直径(count median dianeter,CMD)分别为2.76和3.66μm,粒子均可直接进入肺泡,而且能直接进入肺泡的真菌粒子浓度是普通实验室的3.1倍。结论真菌实验室真菌浓度远高于普通实验室,已被真菌污染,而普通实验室真菌浓度较低,CMD值与居室接近,因此,拥有较好的空气质量。

Objective To detect fungal aerosol distribution in laboratory for biological laboratory safty. Methods Airborne fungal samples were collected by Anderosn 6-stage, PDA mediun in the fungal laboratory and ordinary laboratory. Resuits The predominant fungi genera were A. niger,P, islandiun and A.flavus in the fungal laboratory and 195,60, and 20 CFU/m^3 higher than that of in the average concentration. The prdeominant fungi genera was Penicilliun auounting for 67.4% of fungi in ordinary laboratory. The concentrations of fungal acrosol were 813 CFU/m^3 ,304 CFU/m^3 ,and the CMD （count median dianeter） were 2. 76,3.66μm in fungal and ordinary lobaratory, respectively. Conclusion The results showed that the content of fungi in fungal laboratory was much higher than that of ordinary laboratory.