嗜盐菌Halobacterium species NRC-1不同盐浓度下基因表达差异分析

Analysis on Differential Genes in Changing Salinity in The Halophile Halobacterium species NRC-1

用RNA随机起始PCR(RAP-PCR)技术分析了盐杆菌NRC-1(Halobacterium NRC-1)不同NaCl盐度下基因表达的差异。81条引物用于比较17%、30%两种盐度下基因表达的差异,每条引物平均可以产生10条以上扩增条带,共得到15条在2种盐度下差异表达的条带,这些差异扩增条带的获得将有助从分子水平上了解Halobacterium NRC-1在高盐环境下的适应机制。

The RNA arbitrarily primed PCR （RAP-PCR） approach was used to identify those genes that are differentially expressed in response to changing salinity in Halobacterium NRC-1. 81 primers were used to scan two different RNA pools derived from cultures of 17% and 30% NaC1 concentrations. On average, each RT-PCR reaction yielded more than ten bands on the agrose gel. Of the approximately 900 bands that were generated by 81 RT-PCR reaction pairs, 15 differentially-expressed bands were obtained. The results would be useful to understand the adaptation raeehanism of Halobacterium NRC-1 to high salinity conditions.