实验性腹主动脉瘤中iNOS的分期检测及研究

The assay and investigation of iNOS in experimental abdominal aortic aneurysm

目的:建立实验性腹主动脉瘤(AAA)新模型,探讨腹主动脉瘤中一氧化氮合酶(iNOS)的变化规律及意义。方法:实验组行聚四氟乙烯(PTFE)人工血管材料置入兔肾下腹主动脉修补缝合成瘤手术,分别于术后1d、7d、14d、28d取标本;对照组行兔肾下腹主动脉断流术,并于术后取腹主动脉组织标本;采用酶联免疫吸附法检测兔腹主动脉瘤组织匀浆中iNOS的浓度。结果:实验组兔腹主动脉成瘤术后1d、7d、14d、28d瘤体组织中iNOS的浓度分别为:22.129±2.518u/ml、27.337±5.321u/ml、36.047±4.584u/ml、44.756±1.799u/ml,对照组兔腹主动脉组织中iNOS的浓度为12.499±1.807u/ml;实验组兔腹主动脉瘤瘤体组织中iNOS的浓度均高于对照组(P<0.01);各实验组瘤体组织中iNOS的浓度组间比较,差异均有统计学意义(P<0.05)。结论:iNOS可能在腹主动脉瘤的发生、进展过程中发挥了一定的生物学功能。

Objective： To construct new model of experimental abdominal aortic aueurysm（ AAA） ;To investigate the change and significances of tissue concentration of induce nitric oxide synthase （ iNOS ） in the abdominal aortic aneurysm. Methods：During the operation of the experiment group, the vascular prosthesis of （stuff） PTFE was implanted to the rabbit abdominal aorta to form an aneurysm,while sham operation was done in the control group. The tissue of abdominal aortic aneurysm was harvested in 1 d,7d, 14d,and 28d after operation,respectively. The tissue bomogenate concentration of iNOS in the abdominal aortic aneurysm were detected by enzyme linked immunosorbent assay （ ELISA ） Results：In experimental group, mean concentration of the tissue bomogenate concentration of iNOS in the abdominal aortic aneurysm in 1-day after operation,7-day after operation,14-day after operation,28-day after operation is 22. 129 ± 2. 518u/ml,27. 337 ± 5. 321u/ ml,36.047 ± 4.584u/ml,44. 756 ± 1. 799 u/ml, respectively, In control group, that is 12.499 ± 1. 807u/ml. The concentration of iNOS in experimental group is significantly higher than that of control group, （ P 〈 0.01 ）. The difference during the experimental group all have statistical significance, （ P 〈 0.05 ）. Conelusion： It is possible that iNOS has some biological function during the formation and progression of the abdominal aortic aneurysm.