正交实验优选复方斯亚旦生发酊总皂苷的提取工艺研究

Optimization of extraction process of total Saponin in Compound Siyadan Tincture by orthogonal design

目的:建立复方斯亚旦生发酊剂中总皂苷的提取工艺。方法:以常春藤皂苷元为对照,以总皂苷含量为指标,设计L_93_4正交实验,确定最佳的提取工艺。结果:10倍量95%乙醇提取1次,每次2h为优化提取方案。结论:本工艺条件对复方斯亚旦生发酊总皂苷的提取收率高且稳定,为复方斯亚旦生法酊总皂苷的工业化生产和新药开发提供了依据。

Objective： To establish the extraction technology of total saponin in Compound Siyadan Tincture. Methods：L934 orthogonal test was designed with Hederagenin as the control and content of total saponin to determine the rational extract technology. Results：Ten times quantity of 95% ethanol was used to extract, once, 2 h each time as the best extract plan. Conclusion：The extract rate of total saponin was high and steady with this technology, which provides new evidence for mass production of the plant and development of new drugs.