口腔颌面部病变组织中人乳头瘤病毒DNA的检测

Detection of Human Papilloma Virus DNA in Tissues of Oral and Maxillo-facial Regions Using Polymerase Chain Reaction

为探讨人乳头瘤病毒（ＨＰＶ）和口腔颌面部肿瘤以及某些口腔粘膜病的关系，应用聚合酶链反应技术（ＰＣＲ）检测４型人乳头瘤病毒６，１１，１６，１８型ＤＮＡ．结果：４５例口腔颌面部良性病变和恶性肿瘤患者，４８份组织标本中的人乳头瘤病毒ＤＮＡ（ＨＰＶ－ＤＮＡ），总阳性率达７５．６％（３４／４５）．其中恶性肿瘤ＨＰＶ－ＤＮＡ阳性率为７６．９（２０／２６）．良性病变ＨＰＶ－ＤＮＡ阳性率为７３．７％（１４／１９）．恶性肿瘤和良性病变２组间ＨＰＶ－ＤＮＡ的型别有显著的差异，恶性肿瘤以ＨＰＶ１６和ＨＰＶ１８型为主，良性病变以ＨＰＶ１８和ＨＰＶ６为主．非肿瘤对照组共３０人ＨＰＶ－ＤＮＡ阳性率为１０％，均为ＨＰＶ１８型．ＨＰＶ１８在口腔颌面部恶性肿瘤和良性病变组织中阳性率均较高。

Using Polymerase chain Reaction (PCR),an investigation was made on the presence and characteristic distributions of human papilloma virus DNA(HPV-DNA)types 6,11,16 and 18 in patients with oral and maxillofacial tumors and oral mucous membrane diseases.Forty-eight biopsy specimens from 45 patients were examined.The results were as follows:The reaction was positive for HPV-DNA in 34 of 45 cases(75.6%)including 20 in 26 cases with malignancies(76.9%) and 14 in 19 cases with benign diseases(73.7%).The type-difference was significant between benign and malignant conditions.In malignancies,types 16 and 18 were predominant,whereas in benign cases mainly types 18 and 6 were found.Only HPV-DNA 18 was found in 10% of the 30 controls.the frequency of the presence of HPV-DNA 18 in both benign and malignant cases was rather high.However no statistical difference was found between them.