骨髓干细胞动员疗法对心肌梗死大鼠心肌的促血管生成作用

The stimulate angiogenesis effect of bone marrow stem cell mobilization on the hearts of myocardial infarction rats

目的探讨骨髓干细胞动员疗法对心肌梗死大鼠冠状动脉新生血管形成的影响。方法结扎Wistar大鼠左冠状动脉制作急性心肌梗死（AMI）模型，人参皂甙Rg1联用辛伐他汀动员自体骨髓干细胞，建模后第24h、1周和4周时杀死大鼠，取出心脏，HE染色检测梗死面积，免疫组化方法观察心肌梗死灶、边缘区和正常心肌组织CD34阳性细胞及Ⅷ因子表达。结果人参皂甙Rg1联用辛伐他汀治疗后，治疗组大鼠心肌梗死区可见CD34阳性细胞浸润；治疗组大鼠梗死面积明显减小；治疗组大鼠梗死灶及周围组织中微血管密度明显高于AMI组及假手术对照组。结论在大鼠AMI模型中，人参皂甙Rg1联用辛伐他汀治疗，可动员骨髓干细胞归巢于缺血心肌，有效促进微血管形成，促进缺血心脏功能恢复。

Objective To investigate the stimulate angiogenesis effect of bone marrow stem cell mobilization on coronary collateral development in the hearts of experimental myocardial infarction rats. Methods Left anterior descending coronary arteries were ligated to produce acute myocardial infarction （AMI） model in Wistar rats. Bone marrow stem cells were mobilized and home to the site of myocardial infarction by ginsenoside Rgl and Simvastatin. Hearts were harvested in the 24th hour,1 st and 4th week after AMI modeling for histopathologieal examination. Immunohistochemisty were used to deteet infiltration of CD34^＋eells and the expression of Ⅷ faetor in the part of ischemia. And infarct area were measured. Results Infarct area in mobilized group was obviously less than in AMI group. There were a great number of monocytes infiltrating with CD34 expression by immunohistochemisty in myocardial infracted zone in mobilized rats. Capillary density in mobilized group was greater than those of AMI and sham-operated groups. Conclusion In the AMI model of rat, bone marrow stem cells ean be mobilized by ginsenoside Rgl and Simvastatin. The capillary density can be increased by mobilizing bone marrow stem cells. Ginsenoside Rgl and Simvastatin can improve the acute ischemic cardiac function by enhancing angiogenesis.