尿激酶型纤溶酶原激活物和组织蛋白酶D在宫颈鳞癌及其癌前病变中的表达

Expression and clinical significance of uPA and Cath-D proteins in the diagnosis of cervix carcinoma and cervical intraepithelial neoplasia

目的探讨宫颈上皮内瘤变及宫颈鳞癌中尿激酶型纤溶酶原激活物（uPA）、组织蛋白酶D（Cath-D）的表达及其意义。方法应用免疫组织化学S-P法检测宫颈鳞癌39例、宫颈上皮内瘤变33例、正常宫颈组织15例中uPA、Cath-D的表达水平，并进行分析。结果uPA、Cath—D在正常宫颈组织、宫颈上皮内瘤变组织及宫颈鳞癌中的阳性表达率呈上升趋势（P〈0．05）；uPA、Cath—D在CINⅠ组、CINⅡ组、CINⅢ组中的阳性表达率呈上升趋势（P〈0．05）；uPA、Cath-D在高、中、低分化宫颈鳞癌中的阳性表达率呈上升趋势（P〈0．05）；uPA、Cath-D在无淋巴结转移组中的阳性表达率显著低于有淋巴结转移组（P〈0．01）。结论uPA、Cath-D联合检测作为诊断宫颈癌前病变及宫颈鳞癌的指标，可提高宫颈鳞癌的早期诊断率。

Objective To investigate the expression,and biological significance of uPA and Cath-D in early diagnosis of cervix carcinoma and cervical intraepithelial neoplasia. Methods The immunohistochemical SP was used to detect the expression of uPA and Cath-D proteins in 39 cases with cervix carcinoma and 33 of patients with CIN cases and 15 eases with normal cervical epithelia. Results Positive rates of uPA and Cath-D in the cervix carcinoma were significantly higher than in the CIN. Positive rates of uPA and Cath-D in the CIN and the cervix carcinoma were significantly higher than in the normal cervical epithelia. The difference had statistical significance （P 〈 0. 05 ）. In the group of CIN I/CIN Ⅱ/CINⅢ ,the expression of uPA and Cath-D had significant difference（ P 〈 0. 05 ）. In the group of highly, moderate and lowly developed tissue, the expression of uPA and Cath-D had significant difference （ P 〈 0. 05）. In the group with and without LNM, the expression of uPA and Cath-D had significant difference （P 〈 0. 01 ）. Conclusions uPA and Cath-D may involve in the initiation of CIN, cervix carcinoma, uPA and Cath-D could be used as markers for early diagnosis of cervix carcinoma. It could be helpful for increasing the ratio of earlier diagnosis in the fields of cervix carcinoma.