猕猴桃汁抗环磷酰胺致突变作用的机理

Antimutagenic Mechanism of Kiwifruit Juice

目的用大鼠外周血双核淋巴细胞微核测试法（ＣＢＭＮＴ），在哺乳动物整体水平，研究猕猴桃汁抗环磷酰胺（ＣＰ）的致突变作用以及生物转化Ⅱ相酶的作用。方法测定大鼠外周血双核淋巴细胞微核细胞率及肝组织中总谷胱甘肽硫转移酶（ＧＳＴ）、尿苷二磷酸葡萄糖醛酸转移酶（ＵＤＰＧＴ）、谷氨酰胺转肽酶（γＧＴ）活性。结果猕猴桃汁对ＣＰ诱发的大鼠外周血双核淋巴细胞微核细胞率有显著抑制作用，能明显诱导大鼠肝脏总ＧＳＴ、ＵＤＧＴＰ活性，但对γＧＴ活性无显著影响。大鼠外周血双核淋巴细胞微核细胞率与总ＧＳＴ、ＵＤＧＴＰ活性呈明显负相关。结论猕猴桃汁抗ＣＰ致微核形成作用的机理可能是通过诱导机体外来化合物代谢解毒酶系。

Objective To observe the effects of kiwifruit juice on the mutagenicity induced by cyclophosphamide (CP) and the effects of biotransformation enzymes in rats. Methods We measured the frequency of cells with micronuclei in binucleated lymphocytes and the activities of liver glutathione S transferase (GST), uridine diphosphate glucuronyltransfer (UDPGT), γ glutamyl transpeptidase (γ GT). Results Kiwifruit juice inhibited the frequency of cytokinesis blocked cells with micronuclei (MN) induced by CP in rat peripheral blood lymphocytes, and increased significantly the activity of liver GST and UDPGT. The rate of MN cells was significantly correlated negatively with the activity of liver GST and UDPGT. Conclusion The effects of kiwifruit juice on anti micronucleus formation may be related to the increased activities of detoxidation enzymes.