逆转录病毒介导的反义bcl-2序列促进足叶乙甙诱导的白血病细胞凋亡

Enhancement of Vp16 inducing apoptosis of leukemic cells by retrovirus mediated bcl 2 anti sense RNA

目的：探讨逆转录病毒介导的反义ｂｃｌ２序列对细胞凋亡的诱导作用。方法：ＰＡ３１７细胞包装逆转录病毒，病毒转导Ｊｕｒｋａｔ细胞后用ＲＴＰＣＲ及Ｗｅｓｔｅｒｎ印迹法检测ｂｃｌ２ｍＲＮＡ及蛋白表达水平；用流式细胞仪计数及ＤＮＡ电泳检测细胞凋亡。结果：转染反义ｂｃｌ２序列可使内源性ＢＣＬ２蛋白表达下降，提高白血病细胞株对足叶乙甙（Ｖｐ１６）的敏感性，促进细胞凋亡。结论：反义ｂｃｌ２序列能促进Ｖｐ１６诱导的白血病细胞凋亡，为ｂｃｌ２反义技术治疗白血病的临床应用提供了实验依据。

Objective:To explore the effects of retrovirus mediated bcl 2 antisense RNA on apoptosis of leukemic cells. Methods: Retrovirus was packaged in vitro with PA317 cells and Jurkat cell was transducted with collected virus; The expressions of bcl 2 mRNA and protein were assayed by RT PCR and Western blotting, respectively. Apoptosis was assayed by flow cytometry and DNA “ladder”. Results:Expression of intrinsic bcl 2 was decreased, and the sensitivity of leukemic cells to Vp16 and the apoptosis of leukemic cells line Jurkat cells were enhanced by transfected bcl 2 antisense RNA. Conclusion: Antisense bcl 2 enhances Vp16 inducing apoptosis of leukemic cells. The results provide a useful experimental basis for leukemia therapy.