IFN-γ对IL-2基因转染B16细胞治疗肿瘤的增强作用

Enhancing effect of IFNγ on the therapeutic effect of murine melanoma cells transfected with interleukin2 gene

本实验在完成ＩＬ－２基因转染瘤苗的基础上，探索了重组ＩＦＮ－γ对其效果的增强作用。结果表明，ＩＬ－２基因转染使Ｂ１６细胞成瘤性显著降低；小鼠经ＩＬ－２基因修饰的Ｂ１６细胞免疫后，对接种的亲代Ｂ１６细胞有保护作用；ＩＬ－２基因转染的Ｂ１６细胞对接种亲代Ｂ１６细胞的小鼠有一定的治疗作用，与重组ＩＦＮ－γ联合应用后，治疗作用加强。上述结果表明，佐以合适的重组细胞因子，有可能提高单基因转染瘤苗的作用。

We observed the antitumor effects of B16 cells transfected with murine interleukin2 gene and the enhancing effect of recombinant interferonγ. The tumorigenicity of B16 cells transfected with IL2 gene decreased significantly as compared with parental tumor cells and mock vector transfected tumor cells. Moreover, inoculation of the IL2 gene transfected tumor cells activated cytotoxic T lymphocytes and induced protective immunity against parental B16 cells when combined with recombinant interferonγ, the therapeutic effects were enhanced. It suggested that IL2 gene transfected tumor vaccine combined with recombinant interferonγ might get better therapeutic effect, and it migth represent an approach for the immunotherapy for cancer.