MHC Ⅱ 类基因转染对肿瘤免疫的影响

Effects of MHC class Ⅱ gene transfection on tumor immunogenicity

本文用人ＨＬＡ－ＤＲ３基因转染到小鼠黑色素瘤细胞系Ｂ１６，构建了带有异种ＭＨＣⅡ类基因的Ｂ１６－ＤＲ细胞。对此细胞进行免疫学分析发现，Ｂ１６－ＤＲ细胞的致瘤性下降，免疫原性提高；经过Ｂ１６－ＤＲ细胞免疫的小鼠可经受后续的Ｂ１６细胞的攻击；过继免疫也可以在一定程度上保护小鼠抵抗Ｂ１６细胞的攻击。上述结果提示。

B16DR cells with xenogeneic MHC class Ⅱ gene were constructed by transfecting the human HLADR3 cDNA into the murine melanoma cell line B16. The immunoassay for B16DR showed that the tumorigenicity of B16DR was reduced and the immunogenicity was enhanced, B16DR cells immunization protected the mice from wild type B16, adaptive transfection of the B16DR immunized splenocytes also protected the mice from B16 cells′ attack. These results suggest that MHC class Ⅱ gene transfection is favorable to enhancement of tumor immunogenicity.