人反义c-myc重组逆转录病毒表达载体pLNC-aM2和pLNC-aM3的构建

Construction of two human antisense c-myc recombinant retrovirus expression vectors pLNC-aM2 and pLNC-aM3

ｃ－Ｍｙｃ在血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ＶＳＭＣ）增殖、迁移和表型转换（收缩型→合成型）中起着关键作用。为更好地调研ｃ－ｍｙｃ分子中不同区域的作用效应，了解ｃ－ｍｙｃ的转录调控机制，进而找出最佳的反义封闭靶区域，为ＶＳＭＣ增殖和其他增生性疾病的基因治疗提供依据，我们采用分子克隆技术，分别构建了人ｃ－ｍｙｃ第１、第２和第３外显子的反义逆转录病毒表达载体。本文将介绍第２，３外显子反义载体ｐＬＮＣ－ａＭ２和ｐＬＮＣ－ａＭ３的构建过程。

c-myc plays key roles in the proliferation, migration and phenotype change (contractile type→synthetic type) of vascular smooth muscle cells(VSMCs). In order to investigate the effects of different regions of the cmyc molecule, determine the mechanism of cmyc transcription control, and to find out the best c-myc target region for the gene therapy of VSMC proliferation and other proliferative diseases, we used gene cloning technology to construct human cmyc exon 1, exon 2 and exon 3, three antisense retrovirus expression vectors. In this paper, we introduce the process of constructing the exon 2 and exon 3 antisense vectors pLNCaM2 and pLNCaM3.