急性脑梗死患者血清基质金属蛋白酶-2、基质金属蛋白酶组织抑制因子-2的变化及其意义

Study on serum MMP- 2 and TIMP-- 2 in acute cerebral infarction patients

目的观察急性脑梗死患者血清基质金属蛋白酶-2（MMP-2）及基质金属蛋白酶组织押制因子-2（TIMP-2）水平的变化，探讨其在急性脑梗死的发病机制中的作用及临床意义。方法采用酶联免疫吸附法对56例急性脑梗死患者（CI组）及49例健康时照组（NC组）进行血清MMP-2、TIMP-2测定。结果CI组血清MMP-2水平在发病后呈先增高后降至NC组水平的趋势，病程〈24h、2～5d、28d分别为（46.29±14．37）μg／L、（62．18±12．32）μg／L、（35．72±8．91）μg／L，其中以2～5d时最高，与CI组病程〈24h、28d、NC组比较有显著性差异（P〈0．05）。CI组血清TIMP-2水平在病程〈24h、2～5d、28d分别为（186．14±27．91）μg／L、（160．62±25．49）μg／L、（189．01±33．17）μg／L，其中以2～5d时最低，与CI组病程〈24h、28d、NC组比较有显著性差异（P〈0．05）。结论急性脑梗死患者存在血清MMP-2、TIMP-2水平异常，提示MMP-2、TIMP-2参入脑梗死病理过程。

Objective To observe the alterations of serum matrix metalloproteinase--2 （MMP-- 2） and tissue metalkoproteinase inhibitor--2 （TIMP--2） in acute cerebral infarction patients, and to explore their rote and clinical significance in the development of this disease. Methods The levels of serum MMP--2 and TIMP--2 were determined in 56 acute cerebral infarction patients （CI group） and 49 healthy people （NC group） by ELISA. Results 24 hours, 2 to 5 days and 28 days after occurrence of the acute cerebral infarction, the levels of serum MMP-- 2 in CI group were（46.29 ± 14.37） μg/L, （62.18±12.32） μg/L, and（35.72± 8. 91 ）μg/L respectively; A significant increase in the levels of serum MMP-- 2 was found in CI group 2 to 5 days after occurrence of this disease compared to NC group （P 〈0.05）. The levels of serum TIMP--2 in CI group, however, were significantly reduced 2--5 day after occurrence of this disease compared to NC group （P〈 0.05）. Conclusions MMP--2 and TIMP--2 may contribute the pathologic process of cerebral infarction, and the levels of MMP--2 and TIMP--2 are correlated with the clinical course of this disease.