成人牙周炎治疗前后龈沟液蛋白质的电泳分析

Electrophoresis of proteins in gingival crevicular fluid of patients with adult periodontitis before and after treatment

目的通过分析成人牙周炎患者同一位点基础治疗前后１个月龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕｌａｒｆｌｕｉｄ，ＧＣＦ）中蛋白成分的改变，以期发现能够反映牙周健康及疾病状况的特异蛋白。方法采用滤纸条法收集１２例成人牙周炎患者５６个位点ＧＣＦ样本，以十二烷基磺酸钠聚丙烯酰胺凝胶电泳（ＳＤＳＰＡＧＥ）法分离，ＩｍａｇｅＭａｓｔｅｒＴＭ电泳图象分析仪分析其中的蛋白质。结果治疗后蛋白区带数及非血清源低分子量１２０００，１００００蛋白的丰度及百分含量较治疗前显著减少（分别为Ｐ＜０００１和Ｐ＜００５），而３８０００蛋白百分含量呈增加趋势且与治疗前牙周探诊深度和ＧＣＦ量呈负相关（Ｐ＜００５）。结论分子量为１２０００，１００００的蛋白与牙周炎症有关；

Objective To analyze the overall gingival crevicular fluid (GCF) protein profiles from adult periodontitis (AP) both before and one month after conventional periodontal treatment in expectation to find reliable specific protein markers in GCF for healthy and disease.Methods GCF samples were collected using filter paper strips from 56 sites of 12 AP patients. The proteins of GCF were examined via sodium dodecyle sulphate polyacrylamide gel electrophoresis (SDS PAGE) and analyzed by the Image Master TM Electrophoresis Evaluation System.Results After periodontal treatment, the mean number of protein bands and the abundance and percentage of non serum derived low molecular weight proteins (12 000, 10 000) decreased significantly ( P <0 001 and <0 05 respectively). The percentage of non serum derived 38 000 protein increased and correlated negatively with pocket depth and the volume of GCF before treatment.Conclusion ①the elevation of the 12 000 and 10 000 proteins are associated with the periodontal inflammation;②the elevation of 38 000 protein is associated with recovery.