肿瘤特异基因跟踪检测小儿微量残留白血病的研究

Longitudinal Detection of Minimal Residual Disease of Childhood Leukemia with Tumor Gene-specific Markers

应用 PCR 技术,以 SIL-TAL-1、HRX 基因相合的,和 bcr/ab1以及 PML-RAR_α4种融合基因作为肿瘤特异标志,对7例小儿急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)和16例小儿急性早幼粒细胞白血病(acute promyecytic leukemia,APL)在完全缓解(complete remission,CR)后的微量残留病(minimal residual disease,MRD)作系统的跟踪检测。结果所有的小儿 ALL 和 APL 在 CR 后均存在MRD,MRD 持续检测阴性,提示可能临床治愈,若 MRD 持续阳性或由阴转阳则提示将发生骨髓复发。检测的灵敏度是10^(-4)～10^(-6)。白血病 MRD 的跟踪检测有重要的临床意义。

By using polymerase chain reaction analysis,minimal residual disease (MRD) was vertically and systematically detected in 7 children with acute lymphoblastic leukemia (ALL) with SIL-TAL-1,HRX-AFA and BCR/ABL fusion genes,and in 16 children with PML-RARo fusion gene for tumor specific markers.The results showed that all of the cases had MRD after complete remission.It may be clinically cured when the detection of MRD continuously showed negative.It would develop bone marrow relapse when MRD persisted or reappeared.The detection sensitivities of MRD were 10^(-4)～10^(-6).It is important to detect MRD in clinic.