生长因子的寡核苷酸对成纤维细胞增生的影响

Effect of oligonucleotides of some growth factors on proliferation of fibroblast

目的 :观察一些生长因子有义和反义寡核苷酸对成纤维细胞增生的影响。方法 :采用细胞增生试验。结果 :1酸性成纤维细胞生长因子 (a FGF)的寡核苷酸和表皮生长因子 (EGF)的寡核苷酸以抑制细胞增生为主。2碱性成纤维细胞生长因子 (b FGF)的有义寡核苷酸对成纤维细胞增生有抑制作用 ,但其反义寡核苷酸却有促进成纤维细胞增生的倾向。结论 :此实验提示反义寡核苷酸可以作为药物用于某些疾病的治疗 。

Objective:To study the effect of oligonucleotides of some growth factors on proliferation of fibroblasts.Methods:Cell proliferation assay was used.Results:It was found that sense and antisense oligonucleotides of acidic fibroblast growth factor (aFGF) mainly inhibit the proliferation of fibroblasts.Sense and antisense oligonucleotides of epidermal growth factor (EGF) and the sense oligonucleotides of basic fibroblast growth factor (bFGF) also inhibit the proliferation of fibroblasts.On the contrary,antisense oligonucleotides of bFGF seem to enhance the proliferation of fibroblast.Conclusion:Oligonucleotide may be used as drug to treat some kinds of disease,but it works probably through different mechanism.