直流电联合温热杀伤肺癌培养细胞的实验研究

Experimental study of effect of direct electric current combined with hyperthermia on human lung cancer cells

目的 :研究直流电合并温热对人肺鳞癌培养细胞的生长抑制效果。方法 :采用 L78人肺鳞癌细胞以一定浓度接种于 2 4孔培养板内 ,待细胞铺满后 ,用电化学治疗装置 ,通以直流电。同时利用水浴的方法加温 30 min,处理后继续培养 48h。采用噻唑蓝 (MTT)测定每孔的光密度值 ,计算L78人肺鳞癌培养细胞的生长抑制率 ,并观察形态学改变。结果 :直流电合并 41℃以上的加温组的癌细胞生长抑制率明显高于单纯加热和单纯直流电组 (P<0 .0 1 )。结论 :直流电与温热联合可明显杀伤 L78人肺鳞癌细胞。

Objective:To study the effect of direct electric current combined with hyperthermia on growth inhibition of human lung cancer cells in vitro.Methods:Human lung cancer cell line L78 was cultured in 24 well plates.In order to obtain 0.5,1,2 and 4 C/well,the direct electric current was given.Meanwhile,the plates were put in the thermostat water bath for 30 minutes and the cells were cultured for 48 hr.Then the rate of growth inibition of human lung cancer cells were evaluated by MTT assay.Results:The rate of growth inhibition of human lung cancer cells was significantly higher in group of the direct electric current combined hyperthermia (≥41℃) than that of the direct electric current or hyperthermia alone ( P <0 01).Conclusion:The direct electric current combined hyperthermia was demonstrated to possess synergetic effect on growth inhibition of human lung cancer cell L78.