抗凋亡基因bcl-2在子宫内膜癌的表达及意义

Bcl 2 gene expression in endometrial carcinoma

目的探讨抗凋亡基因ｂｃｌ２与子宫内膜癌的关系。方法应用免疫组化链霉菌－抗生物素过氧化物酶染色法，检测１５例子宫内膜非典型增生及５０例子宫内膜腺癌的石蜡包埋标本中ｂｃｌ２基因的表达。结果子宫内膜非典型增生组织中，ｂｃｌ２蛋白高表达，子宫内膜腺癌中ｂｃｌ２低表达（Ｐ＜０．０５）。在子宫内膜腺癌中，随着临床分期的升高、组织病理分级的降低及肌层浸润的加深，ｂｃｌ２表达阳性率逐渐下降（Ｐ＜０．０５）。结论ｂｃｌ２可能与子宫内膜组织的异常增殖有关，并主要在早期起作用。

Objective To evaluate the relationship between bcl 2 gene and endometrial carcinoma. Methods Immunohistochemical staining was performed on formalin fixed paraffin embedded tissue sections. A monoclonal antibody to the bcl 2 gene product was used, the signal was detected using a streptavidin biotin complex with aminoethylcarbazole. There were 15 cases of endometrial atypical hyperplasia and 50 cases of endometrial carcinoma examined. Results Bcl 2 expression was high in endometrial atypical hyperplasia, whereas bcl 2 expression was low in endometrial carcinoma. In endometrial carcinoma, with progression of clinical stage, decreased histologic grade and deepening of cancer invasion, the positive rate of bcl 2 expression was correspondingly decreased. Conclusion Bcl 2 plays a role in the genesis of endometrial carcinoma especially at the initial stage.