胎盘源间充质干细胞对T淋巴细胞体外增殖的影响

The Effect of Placenta-derived Mesenchymal Stem Cells on T Lymphocytes Proliferation in Vitro

目的从人胎盘组织中分离培养获得间充质干细胞(mesenchymal stem cells,MSCs),并进一步研究其对T淋巴细胞体外增殖的影响。方法首先从胎盘组织中分离培养胎盘间充质干细胞(PMSCs),在体外观测其形态,并通过细胞表面抗原表达、分化潜能等特征进行鉴定;然后将体外分离培养扩增的PMSCs,按照不同比例加入双向混合淋巴细胞培养体系(mixed lymphocyte reaction,MLR)中,共同培养6d后,测定T淋巴细胞的增殖。结果从人胎盘组织中分离培养获得间充质干细胞,具有与骨髓间充质干细胞(BMSCs)极为相似的细胞形态及细胞表面标志,表达CD29、CD44和CD105,不表达CD34、CD45、CD106和HLA-DR,并且还具有与BMSCs相似的跨胚层分化能力,能在一定条件下被诱导分化出神经元样细胞。PMSCs与同种异体混合淋巴细胞共同培养能抑制T淋巴细胞的体外增殖。结论胎盘组织是MSCs的有效来源,PMSCs具有与BMSCs相似的生物学特性及免疫调节机制。它为间充质干细胞的研究提供了又一重要的细胞来源。

Objective To isolate and cultivate mesenchymal stem cells from human placenta tissue, and investigate the effect of MSCs from human placenta tissue on allogeneic T lymphocyte proliferation. Methods Firstly we isolated human placenta-derived mesenchymal stem cells（PMSCs） from placenta tissue, observed their morphology and identified cell surface antigen expression by FACS analysis and their differentiation potency in vitro. The PMSCs were cultivated and expanded in vitro, then added to two-way mixed lymphocyte reaction（MLR） essay, according to different ratio of PMSCs to lymphocytes. T lymphocyte proliferation is measured after 6 days＇ eoculture. Results MSCs isolated from placenta tissue had the similar morphology and cell surface markers with bone marrow mesenehymal stein cells（ BMSCs）. CD29, CD44 and CD105 expressions were found on PMSCs but CD34, CD45, CD106 and HLA-DR were not found. PMSCs also can be induced into neuron-like ceils. In MLR essay, PMSCs can inhibit T＇lymphocytes proliferation in vitro. Conclusion The human placenta tissue is proven to be a useful source of the MSCs. PMSCs had the similar biological character and immuno-regulatoly effect with BMSC, which will provide alternative cell source for the mesenchymal stem cells.