兔急性肾衰竭致心肌、胰腺损伤与中性粒细胞扣押

Myocardium and Pancreas Injury and Neutrophils Sequestration in Rabbits Subjected to Acute Renal Failure

目的观察急性肾功能衰竭(ARF)家兔肾、心肌、胰腺髓过氧化物酶(MPO)活性的变化,探讨中性粒细胞(PMN)扣押在兔ARF致心肌、胰腺损伤的作用机制。方法42只家兔均分为对照组、HgCl2组、甘油组。其中HgCl2组以皮下注射1%HgCl2(1.3ml/kg.bw)、甘油组以肌肉注射50%甘油(10ml/kg.bw)分别复制ARF模型,均分为12、24、48h三个亚组。在不同时间点,所有动物颈总动脉插管放血,测定反映肾功能的生化指标;并制备肾、心肌、胰腺组织匀浆,检测MPO的活性。结果两个ARF模型组的血清BUN、Cre水平均显著高于对照组。两个ARF模型组的肾、心肌、胰腺组织匀浆的MPO活性在多个时间点显著高于对照组。HgCl2组BUN、Cre分别与肾、心肌、胰腺组织匀浆的MPO活性正相关;甘油组BUN、Cre分别与肾、胰腺组织匀浆的MPO活性正相关。结论PMN扣押在ARF发病学中具有重要作用,且是ARF致心肌、胰腺损伤的机制之一。

Objective To explore the mechanism of polymorphonuclear neutrophils （PMN） sequestration induced by acute renal failure （ARF） in myocardium and pancreas injury, we observed the changes of myeloperoxidase （MPO） activity in kidney, myocardium and pancreas of rabbits subjected to ARF. Methods 42 rabbits were randomly assigned to control group, HgCl2 group and glycerinum group. The ARF animal model was established by hypodermic injection 1% HgCl2 with 1.3 ml/kg, bw in HgCl2 group or intramuscular injection 50% glycerinum with 10 ml/kg, bw in glycerinum group respectively and all animals in each group were further divided into 12 h, 24 h and 48 h subgroups with six rabbits in each subgroup. At different time points all rabbits were cannulated respectively to collect blood samples for the examination of biochemical indicators, which can reflect renal function, and then kidney, heart and pancreas were taken out for determination of MPO activity. Results The contents of BUN and Cre in two model groups were obviously higher than those of control group and the MPO activity of renal, myocardium and pancreas homogenates in two model groups were significantly increased than that in control group at diverse time points. Moreover, the contents of BUN and Cre were positively correlated with the MPO activity of renal, myocardium and pancreas in HgCl2 group and have significantly positive correlation with the MPO activity of renal and pancreas in glycerinum group respectively. Conclusion PMN sequestration plays an important role in ARF pathogenesis and is one of the mechanisms that ARF causes myocardium and pancreas injury.