不同耐力训练模式下大鼠骨骼肌钙离子泵和RyR1受体功能的实验研究

An Experimental Study of Function of Calcium Ion Pump and RyR1 Receptor of Rats' Muscle in Different Enduance Training Models

目的:在不同耐力训练的模型下,探讨SD.大鼠骨骼肌肌浆网的钙离子转运功能的变化。方法:采用实验法。实验选用2月龄纯种雄性SD.大鼠34只,随机分组。对实验对象采用小动物跑台进行递增耐力负荷训练,达到耐力训练模型标准后,再采用减量耐力训练,主要减少运动强度训练。使用荧光分光光度计对各组实验大鼠骨骼肌肌浆网Ca2+钙离子泵,RyR1受体进行测定。结果:耐力训练组肌浆网肌浆网钙离子泵、RyR1受体功能呈显著性增加(p<0.01)。4周以上减训钙离子泵、RyR1受体功能显著性下降(p<0.01)。结论:耐力训练增强骨骼肌肌浆网钙泵和RyR1受体的功能。4周及以后减训肌浆网钙泵和RyR1受体的功能性明显下降。

The main aim of the study is to discuss the transportation function of calcium ion in sarcoplasrnic reticulum （SR） of muscle in models of different endttrance trainings, Method： 34 2-month-old pure-blood male SD rats are divided to groups randomly. Increasing endttrance load treadmill exercises are used. Reduced intensity is used when reaching the criteria of endurance training. A fluorescence spectrophotometer technology is used to reflect function of calcium ion pump and RyR1 receptor in SR of muscle in experimental objects. Results ： Function of calcium ion pump and RyR1 receptor in SR of muscle, compare to control group, the endurance group is shown remarkable increase（p 〈 0.01 ）. Over 4 weeks re- duced training, the function of calcium ion pump and RyR1 receptor in SR of muscle is remarkable decreased（p 〈 0.01）. Conclusions： Function of calcium ion pump and RyR1 receptor can increase after endurance training. There is obvious decrease of that after 4-week reduced training.