Os WRKY80基因参与调控水稻抗病反应研究

The disease resistance of rice regulated by OsWRKY80 gene

为进一步了解Os WRKY80基因参与调控的抗病分子机理,对Os WRKY80增强表达转基因水稻和干涉转基因水稻进行稻瘟病抗性检测,Northern杂交分析Os WRKY80基因和PR基因(ZB8、PBZ1)稻瘟病接种0 h和24 h时的表达情况。结果表明:与干涉转基因水稻和未转基因对照相比,增强表达转基因水稻表现出对稻瘟病有较好的抗性。Northern杂交结果显示,在干涉转基因植株中内源Os WRKY80基因的诱导表达被抑制,转基因植株的抗病性与Os WRKY80基因的表达呈一定的正相关性。在干涉转基因植株中均未检测到ZB8和PBZ1的表达,稻瘟病诱导24 h时,未转基因植株和增强转基因植株中均能检测到ZB8、PBZ1诱导型表达,但在增强转基因植株中ZB8、PBZ1的诱导表达丰度要显著高于未转基因植株。这表明Os WRKY80基因可能作为正调控因子参与水稻防卫反应,PR基因的高水平诱导表达导致增强表达转基因植株对稻瘟病的抗性增强。

To further understand the mechanism of disease resistance regulated by OsWRKY80 gene,the resistances of OsWRKY80 overexpression and suppression transgenic plants to rice blast were determined,and the Northern hybridization analysis was performed on the expressions of OsWRKY80 gene and pathogenesis-related（PR ）genes（ZB8, PBZ1）in transgenic plants under normal and induction conditions. The results showed that compared with suppression transgenic plants and nontransgenic control plants, the overexpression transgenic plants revealed a rather good resistance to rice blast. The hybridization results indicated that the inducible expression of endogenous OsWRKY80 gene in suppression transgenic plants was inhibited,and there was a certain positive correlation between the disease resistance of transgenic plants and the expression of OsWRKY80 gene. In the suppression transgenic plants no expression of ZB8 and PBZ1 was detected. At 24 h after the nontransgenic plants and ovexpression transgenic plants were inoculated with the germ of rice blast, the inducible expression of ZB8 and PBZ1 was detected and significantly stronger in the overexpression transgenic plants than in the nontransgenic control ones. The above results indicated that as a positive regulatory factor, OsWRKY80 gene could participate in the defense response of rice, and the high-level inducible expression of PR genes could result in enhanced resistance of transgenic plants to rice blast.