不同CO_2压强对MKN-28细胞黏附侵袭能力的影响

Effects of different CO_2 pressures on adhesive and invasive ability of MKN-28 gastric carcinoma cells

目的探讨不同CO2压强及时程对MKN-28细胞黏附侵袭能力的影响。方法利用密封箱建立体外气腹模型,选用胃癌细胞株MKN-28,分别在9mmHg(1mmHg=0.133kPa)和15mmHg压强下以及常规条件下暴露2h和4h后,用CytoMatrixTM细胞黏附试剂盒检测细胞黏附能力,流式细胞技术(FACScan)检测表达E-钙黏蛋白(E-cadherin)和细胞间黏附分子(Intercellular adhesion molecule-1,ICAM-1)的细胞百分数。另将处理后的MKN-28细胞注入裸鼠腹腔(2×106个细胞/只),每组10只。4周后每组取5只处死,记录腹腔成瘤情况,剩余裸鼠观察生存时间。结果MKN-28细胞株经不同压强与不同时程的CO2处理后,胃癌细胞MKIN-28黏附能力,表达E-cadherin和ICAM-1的细胞百分数MKN-28的腹腔内转移个数,生存天数,裸鼠腹腔种植成瘤数和生存时间各组间均差异均无统计学意义(P>0.05)。结论在不高于15mmHg压强,且不超过4h情况下,不同CO2压强及不同时程对MKN-28细胞株的黏附侵袭能力并无显著影响,亦不增加肿瘤的转移几率。

Objective To study the effects of different CO2 pressures on adhesive and invasive alility of MKN-28 gastric carcinoma cells. Methods With an artificial pneumoperitoneum model in vitro, MKN-28 human gastric carcinoma cells were exposure to CO2 in 3 different pressure groups： 9mmI-Ig, 15 mmHg and control group. The adhesive ability of MKN-28 cells before and after exposed to CO2 was detected by CytoMatrixTM kit. The percentage of cells expressing E-cadherin and ICAM-1 among MKN-28 cells were counted by flowcytometry in vitro. All the MKN-28 gastric carcinoma cells were injected into abdominal cavity of nude mice （2 × 10^6 cells/mouse ） respectively. Five mice of each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept to observe the survival time. Results In the 3 groups, all of the adhesive ability of MKN-28 cells （ cd/m2 ） . the percentage of cells expressing E-cadherin, and ICAM- 1 ; the number of intra-abdominal tumor metastases, the survival time and the tumor metastasis in abdominal cavity of nude mice were no sigmificant difference call （ P 〉 0.05 ） .Conclusions When the pressure is below is below 15 mmHg and for less thqn 4 h, different CO2 pressures and different time intervals do not affect the adhesive and invasive ability of MKN-28 cells in the abdominal cavity, and not increase the chancws of tumonr metastasis.