摘要
为了解检测外周血液癌胚抗原信使核糖核酸(CEA mRNA)在诊断大肠癌血液转移方面的应用价值,应用逆转录-套式-聚合酶链反应技术(RT-NP-PCR)检测血液标本CEA mRNA。结果显示,大肠癌组CEA mRNA的阳性率(68.6%)显著高于对照组(2.2%)(P<0.01)。检测CEA mRNA诊断大肠癌血液转移的特异性为97.8%,灵敏度为68.6%,准确度为83.4%。检测血液中CEA mRNA有助于诊断大肠癌血液转移。
To explore the value of carcinoembryonic antigen（CEA） mRNA in peripheral blood in diagnosis of hematogenous metastasis of colorectal cancer,CEA mRNA in blood sample was detected by reverse transcriptase nested primers polymerase chain reaction.The results showed that the positive rate of CEA mRNA in colorectal cancer groups（68.6%） was notably higher than that in controls（P〈0.01）.The accuracy,sensitivity and specificity for the diagnosis of hematogenous metastasis of colorectal cancer with detection of CEA mRNA marker in blood were 83.4%,68.6% and 97.8% respectively.It suggested that the detection of CEA mRNA in peripheral blood would be useful in the diagnosis of hematogenous metastasis of colorectal cancer.
出处
《标记免疫分析与临床》
CAS
2009年第4期211-213,共3页
Labeled Immunoassays and Clinical Medicine
关键词
血液
癌胚抗原信使核糖核酸
癌胚抗原蛋白
诊断
大肠癌
血液转移
Blood
Carcinoembryonic antigen mRNA
Carcinoembryonic antigen protein
Diagnosis
Colorectal cancer
Hematogenous metastasis
