慢性阻塞性肺疾病患者肺内细胞凋亡的研究

Study on the apoptosis of pulmonary cells in patients with COPD

目的探讨慢性阻塞性肺疾病(COPD)患者肺内细胞凋亡情况,以其为临床治疗提供治疗靶点。方法收集COPD患者和非COPD患者肺泡灌洗液,进行细胞培养,取上清液测定白介素8(IL-8)和肿瘤坏死因子-α(TNF-α)含量,测定细胞凋亡率。结果COPD组细胞培养上清液中IL-8和TNF-α含量明显高于对照组(P<0.05);COPD组肺泡巨噬细胞的凋亡明显高于对照组(P<0.05)。结论COPD患者存在着强烈的炎性反应和细胞凋亡情况,可能是患者治疗靶点。

Objective To investigate the apoptotic state of pulmonary cells in patients with chronic obstructive pulmonary diseases （COPD） in order to provide therapeutic targets for clinical therapy. Methods The alveolar irrigating solutions of COPD patients and non-COPD patients were collected, and the cell culture was performed,then the supernatant liquid was taken out to detect the contents of IL-8 and TNF-α, and the cell apoptosis rate was also detected. Results The levels of IL-8 and TNF-α in the supernatant liquid of cell culture in COPD group were significantly higher than those of control group （ P 〈 0.05 ）. The apeptosis rate of alveolar macrophage in COPD group was significantly higher than that of control group （ P 〈 0.05 ）. Conclusion The patients with COPD exist intensive inflammatory reaction and apoptosis,which may be a novel therapy target.