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甘薯黑斑病菌基因组文库构建 被引量：1

Construction of Genomic DNA Library of Ceratocystis fimbriata by Lambda DASHII Phage
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摘要 通过DNA提取方法对比,采用CTAB/LiCl法提取甘薯黑斑病菌基因组DNA,再利用限制性内切酶BamH I对纯化的甘薯黑斑病菌基因组DNA进行部分酶切,获得大小为15kb左右的DNA片段。将经BamHI酶切消化的Lambda DASH II载体臂与DNA片段相连,然后以大肠杆菌XL1BlueMRA(P2)为寄主细胞,在国内首次成功构建了甘薯黑斑病菌(Ceratocystis fimbriata)基因组文库,所构建基因文库效价为4.12×105pfu/mL。 According to comparison of extraction methods, genomic DNA of Ceratocystis fimbriata was extracted by CTAB/LiCl method. While 15kb DNA fragments were obtained by partial digestion of purified genomic DNA of C. fimbr/ata with restriction enzyme BamH I. Repurified 15kb DNA fragments were ligated with Lambda DASH II Phage DNA vector arms digested by BamH I. And a genomic DNA library of C. fimbr/ata was constructed successfully with XL1BlueMRA（P2） as host cell. The titer of the genomic DNA library was 4.12× 10^5pfu/mL
作者 李忆 蔡辉儒 蒲志刚 张敏 阎文昭
机构地区 四川农业科学院生物技术与核技术研究所 四川农业大学 西南交通大学
出处 《西南农业学报》 CSCD 北大核心 2009年第4期956-959,共4页 Southwest China Journal of Agricultural Sciences
基金 "863"计划(2006AA100107) 国家支撑项目(2007BAD78B01)
关键词 甘薯黑斑病菌 噬菌体 基因组文库 Ceratocystis fimbr/ata Phage Genomic library
分类号 S531 [农业科学—作物学]
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  • 1陈庆河,王源超,郑小波.中国主要稻区稻瘟病菌交配型分布及其育性能力的差异[J].中国农业科学,2004,37(6):840-845. 被引量：9
  • 2肖德全.甘薯黑斑病的发生与防治[J].农业新技术,2005(5):16-16. 被引量：4
  • 3金冬雁 黎孟枫等（译）.分子克隆实验指南[M].北京:科学出版社,1992.885.
  • 4[1]Backhaus R A. Rubber formation in plants -a mini-review. Isrel Journal of Botany, 1985;34:283 - 293
  • 5[2]Bealing I J. Carbohydrate metabolism in Hevea latex -availability and utilization of substrates. J Rubber Res lnst Malays. 1969; 21:445 - 455
  • 6[3]Kush A. Isoprenoid biosynthesis:the Hevea factory. Plant Physiol Biochem, 1994; 32:61 - 67
  • 7[5]Sambrook J, Fritsch E F, Maniatis T. Molecular cloning:a laboratory manual. 2nd. New York:Cold Spring Harbor Laboratory Press, 1989: 75- 166
  • 8J．萨姆布鲁克等 金冬雁（译）.分子克隆实验指南（第二版）[M].北京:科学出版社,1989.1-1062.
  • 9Saghai M A, Soliman K M, Jorgensen R A, et al. Ribosomal DNA spacer-length polymorphism in barley:mendeian inheritance, chromosomal location and population dynamics [J]. Proc. Natl. Acad. Sci. USA,1984, 81(24): 8014-8018.
  • 10He Y Q. An improved protocol for fungal DNA preparation[ J]. Mycosystema, 2000, 19(3) : 434.

共引文献163

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  • 1林翙,方盛国.基因组文库构建及其在保护遗传学中的应用前景[J].兽类学报,2005,25(1):86-90. 被引量：5
  • 2萨姆布鲁克J,拉塞尔D W,黄培堂,王嘉玺,朱厚础,等译.分子克隆实验指南[M]3版.北京:科学出版社,2002..
  • 3ALEXANDER L J, SMITH T P, BEATTIE C W, et al. Construction and characterization of a large insert porcine YAC library [ J ]. Mammalian Genome: Official Journal of the International Mammalian Genome Society, 1997, 8 (1) : 50 -51.
  • 4LEE K T, BYUN M J, LIM D, et al. Full-length en- riched cDNA library construction from tissues related to energy metabolism in pigs [ J ]. Molecules and Ceils, 2009, 28 (6) : 529 -536.
  • 5LIU L, YIN J, LI W, et al. Construction of a bacterial arti- ficial chromosome library for the rongchang pig breed and its use for the identification of genes involved in intramuscular fat deposition [ J]. Biochemical and Biophysical Research Communications, 2010, 391 (2): 1280-1284.
  • 6FRISCHAUF A M, LEHRACH H, POUSTKA A, et al. Lambda replacement vectors carrying polylinker se- quences [J]. Journal of Molecular Biology, 1983, 170 (4) : 827 -842.
  • 7BURKE D T, CARLE G F, OLSON M V. Cloning of larger segments of exogenous DNA into yeast by means of artificial chromosome vectors [ J]. Science, 1987, 236 (4803) : 806 -812.
  • 8SHIZUYA H, BIRREN B, KIM U J, et al. Cloning and stable maintenance of 300-kilobase-pair fragments of hu- man DNA in escherichia coli using an F-factor-based vector [ J ]. Proceedings of the National Academy of Sciences of the United States of America, 1992, 89 (18) : 8794-8797.
  • 9LOANNOU P A, AMEMIYA C T, GARNES J, et al. A new bacteriophage Pl-derived vector for the propagation of large human DNA fragments [ J]. Nature Genetics, 1994, 6 (1): 84-89.
  • 10FARR C J, BAYNE R A, KIPLING D, et al. Genera- tion of a human X-derived minichromosome using te- lomere-associated chromosome fragmentation [ J ]. The EMBO Journal, 1995, 14 (21):5444-5454.

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西南农业学报
2009年 第4期

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