应用组织芯片技术检测CD44v6和Ezrin蛋白在子宫内膜癌中的表达及意义

The measurement of CD44v6 and Ezrin expression by tissue microarrayin in endometrial adenocracinoma and its significance

目的研究CD44v6和Ezrin在不同类型子宫内膜中的表达情况，探讨其在子宫内膜癌发病机制中的意义。方法应用组织芯片技术观察CD44v6和Ezrin在子宫内膜癌（58例）、子宫内膜不典型增生（30例）、子宫内膜单纯性增生及复杂性增生子宫内膜（20例）、正常增生期子宫内膜（20例）中的免疫组化表达。结果CD44v6和Ezrin在正常增生期子宫内膜、单纯及复杂性增生子宫内膜、不典型增生及子宫内膜癌中阳性表达逐渐升高，差异有统计学意义（P〈0．05）。CD44v6表达强度与子宫内膜癌手术分期及有无伴淋巴结转移有关，差异有统计学意义（P〈0.01），子宫内膜癌中Ezrin表达与浸润肌层的深度有关（P〈0．05）。CD44v6蛋白的含量与Ezrin蛋白含量有关。结论CD44v6、Ezrin的过度表达与子宫内膜异常增生及恶变有关，参与子宫内膜癌的发生发展过程。

Objective To investigate the expression of CD44v6 and Ezrin in endometrial carcinoma and its significance. Methods The expression of CD44v6 and Ezrin by the application of tissue microarray in 58 cases of uterine endometrial adenocarcinoma, 30 cases of atypical endometrial hyperplasia, 20 cases of complex endometrial hyperplasias and 20 cases of normal endometrium. Results The positive rates of CD44v6 and Ezrin expression were increased progressively in the order of normal endometrium during proliferative phase, simple hyperplasia, complex hyperplasia, atypical hyperplasia and endometrial carcinoma （P〈0.05）. The expression intensity of CD44v6 was related to the clinical staging and lymphatic metastasis in endometrial carcinoma （P〈0.01）. The expression of Ezrin was significantly related to muscular layer invasion （P〈0.05）. The contents of CD44v6 protein were correlated with those of Ezrin. Conclusion The overexpression of CD44v6 and Ezrin may be associated with the process of the uterine endometrial abnormal hyperplasia, as well as oncogenesis and development of the endometrial carcinoma.