整合素β3mRNA和VEGF表达与胃癌临床病理及预后的相关性

Correlation of integrin β 3 mRNA and VEGF protein expression with clinicopathology and prognosis of gastric carcinoma

目的探讨整合素β3mRNA、血管内皮生长因子（VEGF）在胃癌中的表达及其与胃癌临床、病理和预后的关系。方法采用原位杂交和免疫组织化学技术，检测118例胃癌组织中整合素β3mRNA、VEGF和CD34的表达。结果非肿瘤胃黏膜中整合素β3mRNA的阳性表达率显著低于胃癌组（P〈0．01）；具有浸润性生长、脉管侵犯和淋巴结、肝脏、腹膜转移的T3～T4期胃癌组织中整合素β3mRNA、VEGF阳性表达例数和微血管密度值（MVD）值均显著高于膨胀性生长和无脉管侵犯、淋巴结转移、肝脏及腹膜转移的T1～T1期胃癌组织（均P〈0．01）：整合素β3mRNA、VEGF表达和MVD值之间均呈正相关性（均P〈0．01）；整合素β3mRNA、VEGF呈阳性表达和MVD值≥54.9个／mm^2患者平均生存时间和5年生存率均低于整合素β3mRNA、VEGF呈阴性表达和MVD值〈54.9个／mm^2者（均P〈0．05或0.01）。结论整合素β3和VEGF可促进胃癌血管生成，并参与肿瘤侵袭转移过程，检测整合素β3和VEGF的表达可作为评价胃癌患者临床、病理和预后的重要参考指标。

Objective To investigate the expression of integrin β3 mRNA and VEGF protein in gastric carcinoma, and its correlation with prognosis and clinicopathology of the disease. Methods In situ hybridization and immunohistochemical method was used to examine the expression of integrin β3 mRNA, VEGF and CD34 protein in 118 specimens of gastric carcinoma. Results The positive rate of integrin β3 mRNA in non-tumor gastric mucosa was significantly lower than that in gastric cancer tissue （P〈 0.01）; The positive expression rates of integrin β3 mRNA and VEGF protein and micro vessel density （MVD） were significantly higher in gastric cancer in stage T3-T4 with aggressive growth, vessel invasion, lymphatic metastasis and hepatic or peritoneal metastasis than those in stage T1-T2 with expanding growth, without vessel invasion, without lymphatic, hepatic and non-peritoneal metastasis （P 〈0.01）. Integrin β3 mRNA,VEGF protein and MVD were positively correlated with each other （P〈 0.05）. The mean survival time and five-year survival rate in cases with positive expression of integrin β3 mRNA and VEGF protein, and MVD value≥54.9/mm2 were significantly shorter than those with negative expression of integrin 15 3 and VEGF, and MVD value 〈54.9/mm2 （P 〈0.05 or 0.01）. Conclusion Integrin β3 and VEGF can enhance synergistically tumor angiogenesis and may play a crucial role in invasion and metastasis of gastric carcinoma, which indicates that their expression might be used as markers for prognosis of the disease.