HBVS-ecdCD40L融合基因构建及相应融合蛋白的二级结构预测

Construction of HBV S-ecdCD40L fusion gene and prediction of secondary structure of the fusion protein.

目的构建HBVS—ecdCD40L融合基因，并利用软件对其相应融合蛋白的二级结构进行预测。方法利用分子生物学技术将HBVS基因与人CD40L胞外段基因进行融合，构建融合基因及其真核表达载体，并利用蛋白质分析软件对相应融合蛋白二级结构水平的一些生物学特性进行预测。结果融合基因序列与设计一致，其相应氨基酸序列经软件分析，并与HBsAg和CD40L胞外段氨基酸序列分析结果比较，发现在二级结构上几乎无变化，亲水性和抗原性等生物学活性几乎未受影响。结论HBVS-ecdCD40L融合基因构建成功，软件分析表明融合过程未影响HBsAg和人CD40L胞外段的生物学活性，为进一步研究奠定基础。

Objective To construct the HBV S-ecdCD40L fusion gene and to predict the secondary structure of the fusion protein. Methods The HBV S gene and the cDNA of extracellular domain of human CD40 ligand were linked by using PCR cloning. The protein analysis software was applied to predict the flexibility, hydrophilicity, antigenicity of the fusion protein. Results The software analysis demonstrated that the secondary structure of HBsAg and ecdCD40L was not changed after fusion and remained their biological activities. Conclusion The fusion gene containing HBV S gene and ecdCD40L gene was constructed successfully.