表面活性剂对间苯二胺-H_2O_2-HRP伏安酶联免疫分析体系测定HRP的影响

Micellar Solubilization and Sensitization of Surfactants in Determination of HRP with Coupling Reaction Voltammetric EnzymeLinkedImmunoassay Based on mPhenybenedimineH2O2HRP System

考察了多种表面活性剂对间苯二胺－Ｈ２Ｏ２－ＨＲＰ体系偶合反应伏安法测定辣根过氧化物酶（ＨＲＰ）的影响。结果表明，表面活性剂在一定浓度范围内对反应具有较明显的增敏作用。结合紫外－可见光谱法选择出增敏效果最好的表面活性剂，并对检测范围和检测限进行了考察。结果表明，十四烷基聚氧乙烯（９）醚（ＡＥＯ９）对间苯二胺体系增敏效果最好，浓度达２．０×１０－６ｇ·ｍＬ－１时可使灵敏度提高两个数量级。

The characteristics of the sensitizing effect of surfactants on determination of HRP with coupling reaction voltammetric enzymelinked immunoassay based on MPDH2O2HRP system have been investigated.The surfactants systems are of advantages such as high sensitivity and stability. The best surfactants have been selected by second order derivative linear sweep voltammetry and UV/Visible spectrophotometry.