表面活性剂对伏安酶联免疫分析体系测定HRP的增敏作用

Micellar Solublization and Sensitization of Surfactants in Determination ofHRP with Voltammetric EnzymeLinked Immunoassay

考察了表面活性剂对邻氨基酚－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）、盐酸间氨基酚－Ｈ２Ｏ２－辣根过氧化酶和对氨基酚－Ｈ２Ｏ２－辣根过氧化物酶伏安酶联免疫分析体系测定ＨＲＰ的增敏作用。结果表明：表面活性剂在一定浓度范围内对反应具有增敏作用，并对还原电位有一定影响。据此，可利用加入适当的表面活性剂提高分析方法的灵敏度。同时，对表面活性剂的增敏机理进行了探讨。

The characteristics of the sensitizing effect of surfactants on determination of HRP with voltammetic enzymelinked immunoassay based on aminophenolH2O2HRP system have been investigated in detail. It is indicated that the surfactant systems have some advantages such as high sensitivity and good stability. Some surfactants shift the peak potential. Discussion has been carried out on the reaction that surfactants increase the determination sensitivity and shift the peak potential positively.