应用正交设计法优选黄独脱毒苗快繁培养基

Study on Rapid Propagation Medium for Virus-free Plantlets of Dioscorea bulbifera by Orthogonal Design

通过正交设计法研究黄独脱毒苗的快繁培养基,考察KT、NAA和2,4-D对黄独脱毒苗快繁的影响。结果表明,黄独脱毒苗快繁的最佳培养基为MS+KT2.0mg/L+NAA0.5mg/L。

The rapid propagation medium for virus-free plantlets of Dioscorea bulbifera were studied by orthogonal design, and the effects of rapid propagation medium including different concentrations of KT, NAA and 2,4-D on rapid propagation of Dioscorea bulbifera were also investigated. The results showed that the best rapid propagation medium for virus-free plantlets of Dioscorea bulbifera was MS ＋ KT 2.0mg/L ＋ NAA 0.5 mg/L.