灵芝多糖的结构分析

The Structmral Analysis of Ganoderma lucidum Polysaccharide

从灵芝菌丝体和子实体热水提取液中分离和纯化得到两种多糖，凝胶过滤层析测得分子量分别为３．７×１０４和４．２×１０４。气相色谱分析表明菌丝体多糖含Ｄ－葡萄糖、Ｄ－半乳糖、Ｄ－甘露糖、Ｄ－木糖、Ｌ－岩藻糖、Ｌ－鼠李糖，其摩尔比为５．３５∶２．６７∶１．００∶１．１９∶０．３８∶０．３７；子实体多糖含Ｄ－葡萄糖、Ｄ－半乳糖、Ｄ－甘露糖、Ｄ－木糖、Ｌ－阿拉伯糖、Ｌ－鼠李糖，其摩尔比为５．８２∶２．２３∶１．００∶１．３５∶０．７２∶０．５１。通过ＩＲ、１３Ｃ－ＮＭＲ、高碘酸盐氧化、甲基化分析、Ｓｍｉｔｈ降解、部分酸水解等手段对灵芝多糖的化学结构进行了研究，确定两种多糖都是以β（１→３）糖苷键为主链的杂多糖，在６－Ｏ和４－Ｏ上有分枝。

wo polysaccharides were isolated and purified from the hot water extract of the mycelia and fruiting body of Ganoderma lucidum(GL). Their mean molecular weights were estimated to be approximately 3.7×10 4 and 4.2×10 4 respectively. The components of these polysaccharides were identified by gas chromatography as:D glucose, D galactose, D mannose, D xylose, L fucose and L rhamnose in the molar ratio of 5.35∶2.67∶1.00∶1.19∶0.38∶0.37 in mycelia polysaccharide and D glucose, D galactose, D mannose, D xylose, L arabinose and L rhamnose in the molar ratio of 5.82∶2.23∶1.00∶1.35∶ 0.72 ∶0.51 in fruiting body polysaccharide. By means of IR, 13 C NMR,periodate oxidation, Smith degradation, methylation analysis and partial hydrolysis, etc, the structures of GLP have been identified, and it showed that both had a main chain of β (1→3)linked glucose residues with difference in the branching frequency and linkage mode of side chains.