摘要
首次在汞膜电极上利用循环伏安、微分脉冲和交流伏安法研究脱氧核糖核酸(DNA) 的电化学行为。结果表明汞膜电极作为一类固体电极可应用于负电位区DNA电化学行为的 研究。同时结合凝胶电泳和UV光谱法,研究了用纯高氯酸处理的DNA的氧化还原特性,结 果表明纯高氯酸可引起DNA的变性和降解。纯高氯酸不适宜用于DNA的变性处理。
DNA was studied by means of cyclic voltammetry (CV) with a mercury film electrode (MFE) using conventional CV, differential pulse voltammetry and alternating current voltammetry. The MFE is sufficiently stable and can be used to study electrochemical behaviors of DNA in the negative potential region. This means that MFE is ready to be one kind of solid electrode at which mere useful electrochemical techniques can be carried out, such as spectroelectrochemical techniques. Redos character of DNA treated by pure HClO4 was studied at MFE. It seems that pure HCIO4 would not only bring about the denaturation of DNA but the degradation of it. Pure HCIO4 is not suitable for performing the denaturation of DNA.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第7期819-822,共4页
Chinese Journal of Analytical Chemistry
基金
国家教委资助
国家自然科学基金