P选择素糖蛋白配体-1 VNTR多态性与冠心病的相关性

The variable number of tandem repeat polymorphism in P-selectin glycoprotein ligand-1 gene in relation to coronary heart disease

目的探讨P选择素糖蛋白配体-1（PSGL-1）VNTR多态性与冠心病及其病变严重程度和临床类型间的关系。方法选取行选择性冠状动脉造影的380例患者，其中冠心病组192例，对照组188例（冠状动脉造影阴性）。采用聚合酶链反应检测两组患者PSGL-1 VNTR基因型及等位基因频率，并对其分布进行比较分析。结果（1）PSGL-1 VNTRA、B、C等位基因大小为分别为558、528、498bp，电泳后共发现4种基因型：AA型（558bp一条带）、AB型（558bp和538bp两条带）、BB型（538bp一条带）和BC型（538bp和498bp两条带）。（2）PSGL-1 VNTR基因型以AB型最为多见，其次为AA型，BB型相对较少见，BC型最为少见。等位基因频率分布以A等位基因常见，其次为B等位基因，C等位基因最少见；两组间各基因型及等位基因的分布无明显差异（均P〉0.05）。（3）冠状动脉造影结果示单支病变患者98例，双支病变54例，3支病变40例，3种病变程度冠心病患者各基因型及等位基因的分布无明显差异（均P〉0.05）。（4）心肌梗死43例，不稳定性心绞痛68例，稳定性心绞痛81例，3种不同类型冠心病患者各基因型及等位基因的分布无明显差异（均P〉0.05）。结论PSGL-1 VNTR基因多态性与中国汉族人冠心病无明显相关性。

Objective To investigate the correlation of the variable number of tandem repeat （VNTR） polymorphism in P-selectin glycoprotein ligand-1 gene with the type and the severity of coronary heart disease （CHD）. Methods One hundred and ninety two patients with angiographically confirmed CHD and 188 controls were genotyped for VNTR polymorphism in the P-selectin glycoprotein ligand-1 gene by polymerase chain reaction （PCR） analysis. Results Gel electrophoresis of the PCR products identified 3 bands- 558bp, 528bp and 498bp-which were labeled as alleles A, B and C, and 4 genotypes AA, AB, BB and BC were identified. The frequency of VNTR genotypes was AA （36.6%）, AB （45.5%）, BB （17.6%） and BC （0.3%）; and the frequency of VNTR alleles was A（59.3%）, B（40.5%）, C（0.1%）. There were no significant deferences either in genotype or in alleles between two groups （P〉0.05）. There were 98 patients with 1 pathological branch of coronary artery, 54 patients with 2, and 40 patients with 3; there were no significant differences either in genotype or in alleles among these three groups （P〉0.05）. There were 43 patients with myocardial infarction, 68 patients with unstable angina, and 81 patients with stabile angina; there were no significant differences either in genotype or alleles among these three groups （P〉0.05）. Conclusion No correlation of variable number of tandem repeat potymorphism in the P-selectin glycoprotein ligand-1 gene with CHD was found in the study.