清热燥湿化瘀法对实验大鼠脂质代谢及炎症因子的影响

Effects of heat-clearing, damp-drying and stasis-dissolving method （清热燥湿化瘀法） on lipid metabolism and in flammatory factor of rat with hyperlipemia

目的观察清热燥湿化瘀中药复方预防性给药对实验性高脂血症大鼠血清脂质含量及炎症因子的影响，探讨清热燥湿化瘀法防治动脉粥样硬化的可能机制。方法50只大鼠被随机分为对照组（n=10）、模型组（n=10）、氟伐他汀对照组（西药组，n=15）、清热燥湿化瘀中药复方组（中药组，n=15）。用高脂饲料连续喂养12周制备大鼠高脂血症模型。于第5周开始预防性给予模型大鼠氟伐他汀或中药复方灌胃，连用8周。12周实验结束时取大鼠腹主动脉血，分别测定血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL—C）及C-反应蛋白（CRP）的水平。结果与对照组比较，模型组血清TC、TG、LDL—C、CRP含量均明显升高，HDL—C含量明显降低（P均〈0．01）。中药组血清TC、TG、LDL—C、CRP水平均明显低于模型组，HDL—C明显高于模型组（P均〈0．01），血脂水平与西药组比较差异无统计学意义，但血清CRP水平较西药组下降更明显（P〈0．05）。结论清热燥湿化瘀法方药能有效调节实验大鼠血脂代谢紊乱，抑制炎症因子CRP的水平，改善血管内皮细胞功能，从而发挥抑制动脉粥样硬化的作用。

Objective To observe the effect of preventive administration of composite traditional Chinese medicine （TCM） drugs with heat-clearing, damp-drying and stasis-dissolving （清热燥湿化瘀法） actions on the serum lipid content and inflammatory factor in rats with experimental hyperlipemia, and to approach the possible mechanism of the composite drugs to prevent and treat atherosclerosis. Methods Fifty rats were randomly divided into four groups ： control group （n= 10）, model group （n=10）, fluvastatin group [western medicine （WM）, n = 15], heat-clearing, damp-drying and stasis-dissolving composite prescription group （TCM, n= 15）. The rats were fed with high lipid fodder to prepare hyperlipemia model. Beginning from the 5th week and lasting for 8 weeks in both groups, the composite TCM drugs were intra-gastrically administered into the rat models in the TCM group, and fluvastatin, in the WM group as a preventive measure. At the end of 12th week, the blood in the abdominal aorta was obtained to measure the levels of serum total cholesterol （ TC ）, triglyceride （ TG ）, high-density lipoprotein-cholesterol （ HDL-C ）, low-density lipoprotein-cholesterol （LDL-C） and C-reactive protein （CRP） in various groups. Results Compared with the control group, the serum contents of TC, TG, LDL-C, CRP in the model group were increased obviously, and the content of HDL-C was decreased significantly （all P〈0.01）. The contents of TC, TG, LDL-C, CRP in TCM group were much lower than those in the model group, and the level of HDL-C was much higher than that in the model group （all P〈0. 01）. The comparisons of serum levels of lipids between the TCM group and WM group had no statistical significant differences, but the amplitude of lowering of serum CRP level in the former group was more significant than that in the latter group （P 〈 0.05）. Conclusion Heat-clearing, damp-drying and stasis-dissolving prescription can effectively regulate the lipid metabolic disorder, inhibit the level of inflammatory factor CPR, and improve the vascular endothelial cell function, thus it may play a role of inhibiting atherosclerosis.