心宁片对心肌梗死后大鼠心肌血管内皮生长因子及其受体表达的影响

The influence of Xinning tablets （心宁片 ） on myocardial expressions of vascular endothelial growth factor and its receptor after myocardial infarction in rats

目的观察心宁片对心肌梗死（心梗）后大鼠心肌血管内皮生长因子（VEGF）及其受体胎肝激酶1（FLK-1）表达的影响，探讨心宁片对心梗后大鼠缺血心肌的保护作用及其相关机制。方法采用结扎大鼠左冠状动脉前降支制备心肌缺血模型。将60只SD大鼠随机分为假手术组、模型组、麝香保心丸组（30mg·kg^-1·d^-1）以及心宁片大、中、小剂量组（分别为1．08、0．54和0．27g·kg^-1·d^-1），各组均于制模后2d给药，连用30d。于术前、术后5min、给药30d后动态监测Ⅱ导联心电图，采用免疫组化法测定缺血心肌组织VEGF、FLK-1的表达。结果与假手术组比较，模型组心电图ST段明显抬高，心肌缺血区VEGF及其受体FLK-1的含量显著增加（P〈0．05或P〈0．01）。与模型组比较，心宁片大、中剂量组ST段显著压低[（0．319±0．064）mV、（0．328±0．046）mV比（0．384±0．078）mV，P〈0．01和P〈0．053；心肌缺血区VEGF、FLK-1表达显著升高（VEGFA值：0．190±0．029、0．169±0．027比0．156±0．019；FLK-14值：0．133±0．004、0．101±0．004比0．095±0．003，P〈0．05或P〈0．01]，而心宁片小剂量组虽有压低和升高趋势，但差异无统计学意义。阳性对照药麝香保心丸组与心宁片大、中剂量组的作用差异无统计学意义。结论心宁片可提高心梗后动物缺血心肌组织中的VEGF与FLK-1含量，其中以大剂量组作用最强。

Objective To study the effects of Xinning tablets （心宁片） on the myocardial expressions of vascular endothelial growth factor （VEGF） and its receptor, fetal liver kinase 1 （FLK-1）, and explore the related mechanisms of Xinning tabletsr protective effects on ischemic myocardium in rats after myocardial infarction. Methods The left anterior descending coronary artery was ligated to prepare the rat model with myocardial ischemia. Sixty Sprague-Dawley （SD） rats were divided randomly into sham operation group, model group, Shexiang Baoxin pills （麝香保心丸） positive control group （30mg·kg^-1·d^-1） and Xinning tablets high dose, middle dose, low dose groups （1.08, 0. 54 and 0.27mg·kg^-1·d^-1 respectively）. On the second day after modeling, each treatment group of rats was given respective medicine and the treatment lasted for 30 days. The medicines were also given 5 minutes before and after the operation. After 30 days, the dynamic electrocardiogram I-lead was used to monitor the heart and immunohistochemistry was applied to observe the expressions of VEGF and FLK-1 in the ischemic myoeardium. Results Compared to the sham operation group, the ST segment was elevated obviously and the levels of VEGF and its receptor FLK-1 at the myocardial ischemic region were markedly increased in the model group （P〈0.05 or P〈0.01）. The ST segment in Xinning tahlets high dose and middle dose groups was markedly lower than that in model group [（0.319±0.064） mV, （0.328±0.046） mV vs. （0.384±0.078） mY, P〈0.01 and P〈0.05]; the expressions of VEGF and FLK-1 in ischemic myocardial tissue in the above two Xinning tablets groups were respectively higher than those in model group （VEGF： 0. 190 =k 0. 029, 0. 169 ± 0. 027 vs. 0. 156 ± 0. 019; FLK- 1 ： 0. 133 ± 0. 004, 0. 101 ± 0. 004 vs. 0. 095 ± 0. 003, P 〈 0.05 or P 〈 0.01 ） ; although the Xinning tablets low dose could lower the ST segment and elevate the expressions, but compared to the model group, there were no statistical significances. In the comparisons of actions between the Shexiang Baoxin pills group and high or middle dosage group of Xinning tablets, there were no statistical significant differences. Conclusion Xinning tablets can increase the contents of VEGF and its receptor FLK-1 in myocardial tissue of animals after myocardial infarction, the action of high dose tablets being the strongest.