红花注射液对急性肝损伤大鼠炎症因子的影响

Effect of safflower injection （红花注射液） on inflammatory factors in rats with acute liver injury

目的探讨红花注射液治疗内毒索性急性肝损伤（ALI）大鼠的作用机制。方法将80只SD大鼠随机分为对照组（n=8）、ALI组（n=24）、红花干预组（n＝24）、还原型谷胱甘肽（TAD）组（n=24）。以小剂量脂多糖／D-氨基半乳糖（LPS／D—GaIN）制备大鼠ALI模型，使用红花注射液进行干预，并设TAD注射液为阳性对照组。分别于术后6、24和48h取血和肝组织，测定血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肿瘤坏死因子-α（TNF—α）和白细胞介素-1β（IL—1β）的水平变化；观察肝组织病理学变化和高迁移率族蛋白B1（HMGB1）的表达水平。结果ALI组血清ALT、AST、TNF—α、IL-1β水平均明显高于对照组，且肝组织损伤程度及HMGB1表达也明显高于对照组（P均〈0．01）。红花干预组和TAD组各时间点血清ALT、AST、TNF—α和IL-1β水平明显低于ALI组（P〈0．05或P〈0．01）；肝组织病理损伤程度和HMGB1表达水平也明显低于ALI组（P均〈0．01）。结论红花注射液能够有效抑制内毒素性ALI大鼠炎症因子水平，减轻肝组织炎症反应和水肿、坏死程度，对ALI有较好的防治作用。

Objective To investigate the effects of safflower injection （红花注射液） on changes of inflammatory factor levels in rat models with acute liver injury （ALI） induced by endotoxin. Methods Eighty rats were randomly divided into four groups： ALI model group （n = 24）, safflower injection （H） group （n=24）, reduced glutathione injection （TAD） group （n=24） and normal group （n= 8）. The rat models with ALI were induced by injecting small dosage of lipopolysaccharide/D-galactosamine （LPS/D-Ga1N）; the rats in the H group were injected safflower injection 30 minutes before the establishment of ALI; the rats in the TAD group were injected TAD 30 minutes before ALI; normal saline was injected in the rats in normal group. Eight rats in each group were sacrificed at 6, 24 and 48 hours time points, then their sera as well as hepatic tissues were collected to determine alanine aminotransferase （ALT）, aspartate transaminase （AST）, tumor necrosis faetor-α （TNF-α） and interleukin-1β （IL-1β） levels in the serum and high mobility group box 1 （HMGB1） expression and the pathological changes in liver tissues. Results Compared with the normal group, the serum levels of ALT, AST, TNF-α, IL-1β, the expression of HMGB1 and the pathological changes in liver tissues were significantly elevated at three time points after ALI in ALI group （all P〈0.01）. Compared with ALI group, serum ALT, AST, TNF-α and IL-1β levels, HMGB1 expression and the pathological changes in liver tissues were markedly reduced in H group and TAD group （P〈0.05 or P〈0.01）. Conclusion Safflower injection can effectively inhibit the levels of inflammatory factors in rats with endotoxin ALI, and obviously ameliorate the inflammatory reaction, edema and necrosis in the liver tissues. So safflower injection has relatively good protective and therapeutic effect on ALI.