醋酸纤维素膜固定化脲酶的研究

Studies on Immobilization of Urease on Cellulose Acetate Membrane

酶固定膜反应器兼具有反应和分离两种功能，是酶工程领域中较活跃的研究课题［１～３］．随着酶固定化技术和水平的提高，各种固定化酶生物反应器不断涌现，其中以采用固定化脲酶技术制作的人工肾最为成功［３］．在国内，采用分子筛和聚乙烯醇高含水胶作载体固定化脲酶已...

Urease was immobilized on cellulose acetate membrane by entrapment. The activity yield and stability of urease immobilized membrane were investigated in catalyzing hydrolysis of urea. The experimental results showed that the activity of urease membrane was related to water content of casting membrane solution. After the enzymatic membrane was used repeatedly for 10 times, the residual activity of urease on the membrane retained 60% of its original. After storage at 4 ℃ for 3 months almost no decrease of enzymatic activity was observed. The optimal pH and temperature of the hydrolysis reaction were also studied. The results showed that this urease immobilized membrane can be used under a wide operational condition.