摘要
目的建立清肺饮的质量控制方法。用HPLC法测定清肺饮中哈巴俄苷的含量。方法采用SinochromODS C18柱,以乙腈-水梯度洗脱;流速为1.0ml·min^-1;检测波长为278nm。结果哈巴俄苷在12.37~61.85mg·L^-1.范围内线性关系良好,r=0.9999;平均加样回收率为98.42%,RSD为1.23%(n=5)。结论本法专属性强,准确度高,重现性好,可作为该产品质量控制的方法。
Aim A method was established for the determination of harpagosid in qingfeiyin by HPLC. Method The separation was performed on SinochromODS C18 column, The mobile phase was acetonitrile-water solution in linear gradient elution. The detection wave- length was 238 nm. The flow rate was 1.0 ml · min^-1. Result The linear range of harpagosid was from 12.37 - 61.85 mg· L^-1 ( r = 0. 999 9). The average recovery was 97.86%, and RSD was 1.42 % ( n = 5). Conclusion The method is simple and accurate. It can be used for the quality control of qingfeiyin.
出处
《安徽医药》
CAS
2009年第10期1186-1187,共2页
Anhui Medical and Pharmaceutical Journal