HPLC法测定复方卡托普利片中两种成分的含量

Determination of Two Components in Compound Captopril Tablets by HPLC Methond

采用HypersilCN（150mm×4．6mm，5μm）色谱柱，以甲醇一四氢呋喃一水（甲醇：四氢呋喃：水=10：10：80，磷酸调pH=2．7）作为流动相，测定复方卡托普利片中卡托普利和氢氯噻嗪两种成分的含量．结果表明，卡托普利在50～250μg/ml范围内、氢氯噻嗪在26～130μg/ml范围内线性较好．卡托普利的平均回收率为100．7％，RSD=0．5％（n=5），氢氯噻嗪的平均回收率为100．5％，RSD=0．3％（n=5）．该方法快速简捷、可靠、准确度高、重现性好，可同时测定复方制剂中两种成分．

This research establishes an HPLC method for the determination of Captopril and Hydrochlorothiazide in compound Captopril Tablets. Determination is carried out on a Hypersil CN （150mm×4.6mm,5μm） Column with methanol-water as mobile phase and flow rate is 1.0 ml/min. Detection wavelength is at 220nm. Two good linearity are demonstrated by equation C = 1. 338 ×10 ^-4A - 2. 563 （ n = 5, r = 0.9999） of Captopril and C = 1. 804 ×10 ^-5A - 1. 997 （n = 5, r = 0. 9999） of Hydrochlorothiazide. The average recoveries of Captopril and Hydrochlorothiazide are 100.7% RSD = 0.5% （ n = 5 ） nd 100.5% RSD = 0.3% （ n = 5 ） seperately. This method is simple, reliable and accurate for simultaneous determination of Captopril and hydrochlorothiazide.