温阳益气平喘方对支气管哮喘大鼠组织病理学的影响及Th1／Th2失衡的调节作用

Effects of Wenyang Yiqi Pingchuan recipe （温阳益气平喘方） on Th1/Th2 imbalance and histopathoiogy in bronchial asthmatic rats

目的观察温阳益气平喘方对支气管哮喘大鼠肺组织病理学的影响及辅助性T细胞Th1／Th2失衡的调节作用。方法60只SD大鼠随机分为正常对照组、模型组、温阳益气平喘高剂量组、温阳益气平喘低剂量组、桂龙咳喘宁组、氨茶碱组6组，每组10只。以卵蛋白致敏并喷雾激发制备大鼠支气管哮喘模型，各治疗组均从第1次哮喘激发开始（制模第3周）至处死前每日灌胃给药，4周后处死大鼠，观察肺组织病理学变化，用酶联免疫吸附法（ELIsA）检测肺组织白细胞介素-4（IL-4）和γ-干扰素（IFN-γ）含量及IFN-γ／IL-4比值变化。结果与正常对照组比较，模型组肺组织IL-4明显升高（P〈0.01），IFN-γ有所升高，但差异无统计学意义，IFN-γ／IL-4比值明显降低（P〈0.01）。与模型组比较，各治疗组均可明显减少支气管哮喘大鼠肺组织嗜酸粒细胞，降低IL-4含量，升高IFN-γ含量及IFN-γ／IL-4比值（P〈0.05或P〈0.01），其中以温阳益气平喘高剂量组尤为显著，且明显优于氨茶碱、桂龙咳喘宁组（P〈0.05或P〈0.01）。结论温阳益气平喘方可通过提高IFN-γ／IL-4比值，恢复Th1／Th2失衡，从而调节免疫功能，减轻嗜酸粒细胞对气道的浸润，控制气道炎症。

Objective To investigate the effect of Wenyang Yiqi Pingchuan recipe （WYYQPC,温阳益气平喘方） on helper T lymphocyte Th1/Th2 imbalance and histopathology in bronchial asthmatic rats. Methods Sixty Sprague-Dawley （SD） rats were randomly divided into six groups： the normal control group, the model group, WYYQPC high-dose group, WYYQPC low-dose group, Guilong Kechuanning （桂龙咳喘宁） group, aminophylline group, 10 rats being in each group. The bronchial asthmatic model was established by egg protein sensibilization and long-term spraying inhalation provocation. The rats of each treatment group were given intra-gastric administration each day from the first time of provocation （3 weeks after the beginning of model establishment） to execution. After 4 weeks of treatment, the rats were killed and lung tissues were taken and stained with hematoxylin and eosin for histopathological examinations. The contents of interleukin-4（IL-4） and interferon-γ（IFN-γ） in lung tissues were determined by enzyme linked immunosorbent assay （ELISA）, and the changes of IFN-γ/IL-4 ratio were investigated. Results Compared with the normal control group, in the model group, the content of IL-4 was increased significantly in the lung tissues （P〈0.01）, the content of IFN-γ was increased slightly, but there was no statistical significance, IFN-γ/IL-4 ratio was decreased significantly （P〈0.01）. Compared with the model group, the number of eosinophilic granulocytes and the content of IL-4 were decreased significantly, the content of IFN-γ and IFN-γ/IL-4 ratio were increased significantly in each treatment group （P〈0.05 or P〈0.01）. The improvement in the WYYQPC high-dose group was more obvious, and its effects were more obvious than those in the treatment groups of aminophylline and Guilong Kechuanning （P〈0.05 or P〈0.01）. Conclusion The WYYQPC recipe can elevate IFN-γ/IL-4 ratio, and adjust Th1/Th2 imbalance, thus it may regulate the immune functions, ameliorate the eosinophilic infiltration in the respiratory tract and control the airway inflammation.