摘要
目的研究TNF-α在创伤性休克大鼠多脏器中的表达变化,探讨TNF-α在创伤性休克发生发展过程中的意义。方法取健康成年SD大鼠56只,体质量200~300g,雌雄各半,随机分为对照组,复苏组和休克1、2、4、8、24h组,每组8只,观察各组的死亡率,分别采用RT-PCR法及免疫组化法测定各组肝、脑组织的TNF-αmRNA和肝、脑、肺、肾组织的TNF-α蛋白表达水平。结果对照组和复苏组大鼠均无死亡,休克1h组死亡率为1/8,2h和4h组死亡率为2/8,8h组死亡率为4/8,24h组死亡率为5/8;TNF-αmRNA表达在休克后2h明显升高,肝组织TNF-αmRNA表达水平高于脑组织(p〈0.05);肺、肝、肾组织的TNF-α蛋白表达在休克1h后即开始升高(P〈0.05),脑组织TNF-α蛋白表达水平较低,趋势不明显,但复苏组的肝、脑组织的TNF-αmRNA和蛋白表达较对照组无明显升高(P〉0.05)。结论创伤性休克早期以低血容量表现为主,主要不是由TNF-α介导的;而后期则与TNF-α水平升高相关。
Objective To observe the difference of TNF - α expression in diverse organs of trauma shock rats and investigate the significance of TNF - α during this kind of shock. Methods The normal Sprague - Dawley rats were divided randomly into seven groups of eight per group, which included controls, resuscitation rats, 1 h, 2 h, 4 h, 8 h and 24 h shock rats. The mortality of all groups was assessed. The reverse transcriptase polymerase chain reaction (RT - PCR ) and immunohistochemistry were respectively used to investigate the transcription and expression of TNF -α in liver, brain, lung and kidney. Results The eontrols and resuscitation rats had no death, and the mortality of 1 h, 2 h, 4 h, 8 h and 24 h shock rats were 12. 5% , 25% , 25% , 50% and 62. 5%. The TNF - α transcription increased significantly in liver and brain of 2 h shock rats, and the one was more obvious in liver(P 〈 0. 05 ). The TNF - α protein level began to increase in lung, liver and kidney ( P 〈 0. 05 ) , but not brain in 1 h shock rats. The transcription and expression of TNF - α in liver and brain had no differences between resuscitation and control rats( P 〉 0. 05 ). Conclusion Hypovolemia is the main manifestation in the early stage of trauma shock, which not mediated by TNF - α, while TNF - α plays a part in the later stage of the shock.
出处
《中国急救医学》
CAS
CSCD
北大核心
2009年第9期812-815,I0001,共5页
Chinese Journal of Critical Care Medicine