肠易激综合征患者肠黏膜5-羟色胺的表达及意义

Difference of 5 - hydroxytryptamine expression in intestine of each subgroup of irritable bowel syndrome and its pathological mechanism

目的检测肠易激综合征（IBS）患者肠黏膜5-羟色胺（5-HT）表达的差异，探讨外周5-HT在IBS发病中的作用。方法选择IBS腹泻型（D—IBS）21例、便秘型（C—IBS）22例患者及正常对照组19例，利用免疫组化方法检测各组回肠、近端结肠、远端结肠黏膜5-HT阳性细胞，计数阳性细胞数量并进行分析。结果D—IBS组回肠、近端结肠、远端结肠5-HT阳性细胞数显著多于对照组（P〈0．05），并且回肠黏膜阳性细胞数也多于C—IBS组（P〈0．05），C—IBS组回肠、近端结肠、远端结肠阳性细胞显著多于对照组（P〈0．05）。结论肠道5-HT的增加与IBS发病相关，IBS不同亚型发病机制可能与5-HT不同受体在中枢神经系统和消化道表达分布有一定关系。

Objective To investigate the role of 5 -hydroxytryptamine （5 -HT）in patients with irritable bowel syndrome （IBS）. Methods By immunohistochemical assay, the 5 - HT levels were detected in intestinal mucosa in control group, D - IBS （ diarrhea IBS） group and C - IBS （ constipation IBS） group. Results The number of 5 - HT positive cell at ileum, proximate colon, distal colon were significantly more than that of control group, and the number of 5 - HT positive cell at ileum was even more than C - IBS group. The number of ：i - HT positive cell at ileum, proximate colon, distal colon in C - IBS were higher that of control. Conclusions The enchanced 5 - HT in intestine is related to the IBS pathological mechanism, while the occurrence of different IBS subgroups is probably related to the difference expression or distribution of receptors of 5 - HT in center nerve systerm and gut.