摘要
目的探讨Graves病患者甲状腺功能与机体的细胞免疫间的关系。方法（1）采用流式细胞仪检测20例高功能Graves病患者和20例健康对照组的T淋巴细胞亚群和CD8-／IFN-γT（Th1）细胞和CD8-／IL-4＋T（Th2）细胞的百分含量。（2）化学发光法检测FT3、FT4、s-TSH，放射免疫法检测TRAb、TGAb、TMAb。（3）应用逐步回归分析，以FT3、FT4、8-TSH为因变量，CD4T细胞、CD8T细胞、CD4＋／CD8＋T细胞比值、Th2细胞百分含量、Th1细胞百分含量、Th1／Th2比值、TRAb、TGAb、TMAb滴度为自变量进行分析。结果（1）Graves病患者的FT3,s—TSH、TRAb、TGAb、TMAb滴度均较正常对照组明显增高（P〈0．01）。（2）Graves病患者较正常人群CD4＋T细胞和CD4＋／CD8＋T细胞比值增高；Th2百分含量增高；而CD8＋T细胞、Th1细胞及Th1／Th2细胞比值下降（P均〈0．05）。（3）CD4／CD8＋T细胞比值、Th1／Th2细胞比值以及TRAb、TGAb、TMAb成为影响FT3、FT4、TSH变化的重要因素。结论Graves病患者机体的T细胞免疫调节失衡不仅参与疾病的发生和发展，还影响了甲状腺激素功能。CD4＋／CD8＋T细胞比值、Th1／Th2比值可作为临床治疗的监测高功能Graves病活动及疗效的指标。
Objective Study the correlation between thyroid function and cellular immunity in patients with Graves＇ hyperthyroidism （GD）. Methods （ 1 ） T lymphocyte subsets, IFN-γ （ Thl ） T cells and IL-4＋ （Th2） T cells were assayed by flow cytometry from the patients with Graves＇ disease （ n = 20 ） and health control （ HC ） （ n = 20）. （ 2 ） FT3, FT4, s-TSH were assayed by chemoluminescence, and TRAb, TGAb,TMAb were assayed by radioimmunity technique. （ 3 ） Studied the correlation between the thyroid function and cellular immunity in patients with Graves＇ disease by gradual regressive analysis in statistic. Results（ 1 ） FT3, FT4, TRAb., TGAb, TMAb in Graves＇ hyperthyroidism were higher （ P 〈 0.01 ）, but s- TSH was lower. （2）CD4＋ T cells, Th2 cells and the ratio of CD4/CDs cells were higher than those of health control,but CDs T,Thl cells cells and the ratio of Thl/Th2 in GD were lower. （P 〈 0.05 ）. （3） The ratio of CD4/CD8 cells and Thl/Th2 ,TRAb,TGAb,TMAb were important impact factors to the thyroid function. Conclusion These results indicate that the balance of T lymphocyte subset might play a dominant role in the pathogenesis and function in Graves＇ hyperthyroidism. Thus suggest the ratio of CD4＋/ CD8＋ ceils subsets ,the ratio of Th1/Th2 subsets maybe potentially as clinical parameters for disease activity and therapeutic effect.
出处
《临床内科杂志》
CAS
2009年第9期599-602,共4页
Journal of Clinical Internal Medicine
