心钠素基因T2238C多态性和C型受体基因A-55C多态性与老年高血压病的关系

The relationship between endothelial atrial natriuretic peptide and type-C natriuretic peptide receptor gene polymorphisms and essential hypertension in elderly

目的探讨心钠素（ANP）基因T2238C多态性及其C型受体（NPRC）基因A-55C多态性与老年高血压病的关系。方法采用基因芯片技术测定高血压病患者（238例）和健康对照者（184例）的ANP基因T2238C、NPRC基因A-55C多态性，并对两组检测结果进行基因型和等位基因频率的对照观察，应用logistic回归分析基因多态性对血压的影响。结果ANP基因T2238C基因型及等位基因频率在高血压病组与对照组比较差异均有统计学意义（χ^2=4．240～4．728，P均〈0．05）；两组间NPRC基因A-55C基因型和等位基因频率比较差异也有统计学意义（χ^2=5．517～5．950，P均〈0．05）。logistic回归分析显示ANP基因T2238C、NPRC基因A-55C是高血压病发病的危险因素（P〈0．05）。结论ANP基因T2238C和NPRC基因A-55C可能是高血压病的遗传易感基因。

Objective To investigate the relationship between Atrial natriuretic peptide gene T2238C polymorphisms and Type-C natriuretic peptide receptor gene A-55C polymorphisms and essential hypertension in elderly. Method A case-control study was performed to study the association between ANF gene T2238C and NPRC gene A-55C polymorphism and hypertension by gene chip technology. The genotype and allele frequency distribution were compared between groups. Results The frequencies of TT TC genotypes at ANF gene T2238C were 92.4% ,7.6% , in hypertensive subjects and 97.3% , 2.7% , in normotensive subjects（χ^2 = 4. 728, P 〈 0.05 ）. The frequencies of T, C alleles at ANF gene T2238C were96.4% ,3.6% in hypertensive subjects and 98.6% , 1.4% in normotensive subjects （ χ^2 = 4. 240, P 〈 0.05 ） ;The frequencies of CC,AC genotypes at NPRC gene A-55C were 97.5% ,2.5% in hypertensive subjects and 92.4% ,7.6% in normotensive subjects（χ^2 = 5. 950 ,P 〈 0.05 ）. The frequencies of C ,A alleles at NPRC gene A-55C were 98.7% ,1.3% in hypertensive subjects and96.2% ,3.8% in normotensive subjects（ χ^2 = 5. 517, P 〈 0.05 ）. Logistic regression analysis implies relationship between ANF gene T2238C genotypes and NPRC gene A-55C genotypes and esseneial hypertension （P 〈 0.01 ）. Conclusion Our data suggest that ANF gene T2238C variant and NPRC gene A-55C variant are major risk factors for essential hypertension in China.