唐菖蒲复色花突变体AFLP及SCAR标记研究

AFLP and SCAR Markers Analysis on the Dual Color Flower Mutants of Gladiolus

通过AFLP分子标记方法,应用16个引物组合对唐菖蒲M3突变体和对照株进行基因组序列多态性检测,发现有4对引物扩增出稳定的重复性好的多态性条带,4条特异带暂命名为E4M7304、E6M3202、E6M8258和E8M5268。其中E6M8258和E8M5268为对照缺失条带,其他2条为M3缺失条带。将这些特异性条带回收、克隆、测序,设计SCAR引物进行单株验证,其中E4M7304标记已经转换成了稳定的SCAR标记。由于唐菖蒲从子球到开花种球的繁殖年限较长,故可利用该SCAR标记对唐菖蒲的花色变异突变体后代进行早期分子鉴定,以提高育种效率。

16 pairs of AFLP （amplified fragment length polymorphism） primer were used to examine the polymorphism between gladiolus M3 and control. 4 of 16 selective primer pairs amplified stable and reproducible polymorphic products. 4 polymorphic fragments were named E4M3 268, E4M7 304, E6M8 258 and E8M5 268 temporarily. The band-E6M8 258 and band-E8M5 268 were absent in the control, and other 2 bands were absent bands of M3. After the cloning and sequencing of 4 polymorphic bands, E4M7304 was transferred into stdble sequence characterized amplified region （SCAR） marker. This marker could be used to Pre-identificate the offsprings of the mutants, which will Increase the efficiency of gladiolus breeding.